Morphologic changes in the outflow pathways of bovine eyes treated with corticosteroids.
Morphologic changes in the outflow pathways of bovine eyes treated with corticosteroids.
复制标题
用皮质类固醇治疗的牛眼流出途径的形态变化。
DOI：
10.1167/iovs.09-4742
复制
发表时间：
2010
影响因子：
4.4
通讯作者：
Lütjen-Drecoll,Elke
中科院分区：
文献类型：
--
作者：
Tektas,Ozan-Yüksel;Hammer,ChristianManfred;Danias,John;Candia,Oscar;Gerometta,Rosana;Podos,StevenM;Lütjen-Drecoll,Elke
目的：分析局部用强的松龙处理后眼压升高的牛眼的小梁网(TM)的形态变化。方法：对4只用0.5%强的松龙滴眼液滴眼7周的成年Braford牛眼和用人工泪液处理的对侧眼，以及2只未用药的成年Braford奶牛和未用药的小牛眼的小梁网(TM)进行光镜和电子显微镜观察。定量评估流出环下细胞外基质(ECM)的增加。此外，用抗VI型胶原抗体标记治疗眼脱离石蜡组织进行免疫细胞化学。结果：在类固醇治疗眼中，ECM(斑块)聚集在流出环内壁的内皮下。在电子显微镜下，这种材料含有标记为VI型胶原的细小纤维。在实验动物的对侧也可以看到斑块，但是斑块的数量明显减少。在未经治疗的Braford对照组和未经治疗的小牛眼中，斑块几乎消失。在治疗眼的TM细胞中，细胞质中的糖原丢失，基底膜样物质增加。这些变化在对侧或未经治疗的动物眼中未见。结论：治疗眼中ECM的积聚与原发性开角型青光眼和激素性青光眼的形态变化相似。因此，该动物模型为进一步研究青光眼TM改变的发病机制提供了一个很好的工具。
Purpose.: To analyze morphologic changes in the trabecular meshwork (TM) of bovine eyes treated with topical prednisolone and exhibiting elevated intraocular pressure for 4 weeks.Methods.: The TM of four adult Braford cow eyes treated with 0.5% prednisolone eye drops three times daily for 7 weeks and their contralateral eyes treated with artificial tear preparation and that of two adult untreated Braford cows and untreated young calves eyes were analyzed with light and electron microscopy. Increased extracellular matrix (ECM) under the outflow loops was evaluated quantitatively. Additionally, deparaffinized tissue of treated eyes was labeled with an antibody against type VI collagen for immunocytochemistry.Results.: In steroid-treated eyes ECM (plaques) accumulated under the endothelium of the inner wall of the outflow loops. On electron microscopy, this material contained fine fibrils that labeled for type VI collagen. Plaques were also seen in the contralateral controls of the treated animals but here they were significantly less in amount. In the untreated Braford controls and in untreated calf eyes, plaques were nearly absent. In the TM cells of the treated eyes there was a loss of glycogen from the cytoplasm and an increase in basement membrane-like material. These changes were not seen in contralateral eyes or eyes of untreated animals.Conclusions.: Accumulations of ECM in the treated eyes resembled morphologic changes in human eyes with primary open-angle glaucoma and steroid-induced glaucoma. This animal model, therefore, provides a good tool in which to further study the pathogenesis of TM changes in glaucoma.