FORWARD TRANSPORT OF GLYCOPROTEINS ON LEADING LAMELLIPODIA IN LOCOMOTING CELLS
FORWARD TRANSPORT OF GLYCOPROTEINS ON LEADING LAMELLIPODIA IN LOCOMOTING CELLS
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DOI：
10.1038/340315a0
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发表时间：
1989-07-27
期刊：
影响因子：
64.8
通讯作者：
SHEETZ, MP
中科院分区：
文献类型：
--
作者：
KUCIK, DF;ELSON, EL;SHEETZ, MP
在几种类型的运动细胞中，已观察到细胞表面颗粒的主动向后运输并与运动性相关1-4。然而，此前尚未报道过颗粒的向前传输。在这里，我们报道了鱼表皮角质细胞板状伪足背表面上伴刀豆球蛋白 A 包被的金颗粒的快速向前运输。这些运动是主动的，而不是扩散的，并且比向后的粒子传输或细胞运动的速率更快。我们观察到迁移细胞中的向前运输，但在静止细胞中没有观察到，并且可以通过细胞松弛素 D 处理来阻止移动。这些研究首次证明少量糖蛋白可以在细胞表面主动运输到板状足的前部。我们认为这种机制运输参与细胞运动的蛋白质，例如粘附所需的蛋白质，并且还可以产生拉伸力。
IN several types of locomoting cells, active rearward transport of particles on the cell surface has been observed and correlated with motility1-4. No forward transport of particles has previously been reported, however. Here we report rapid forward transport of concanavalin A-coated gold particles on the dorsal surfaces of lamellipodia of fish epidermal keratocytes. These movements are active, not diffusive, and more rapid than either rearward particle transport or the rate of cell locomotion. We observed forward transport in migrating, but not in stationary cells, and could block the movement by treatment with cytochalasin D. These studies demonstrate for the first time that small numbers of glycoproteins can be actively transported on the surface of the cell to the front of the lamellipodium. We suggest that this mechanism transports proteins involved in cell locomotion, such as proteins necessary for adhesion, and could also produce an extensile force.