Development of a recombinant enzyme-linked immunosorbent assay for detection of antibodies against Epstein-Barr virus nuclear antigens 2A and 2B.
Development of a recombinant enzyme-linked immunosorbent assay for detection of antibodies against Epstein-Barr virus nuclear antigens 2A and 2B.
复制标题
开发重组酶联免疫吸附测定法，用于检测 Epstein-Barr 病毒核抗原 2A 和 2B 的抗体。
DOI：
10.1128/jcm.32.1.112-120.1994
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发表时间：
1994
影响因子：
9.4
通讯作者：
Rymo,L
中科院分区：
文献类型：
--
作者：
Geertsen,R;Espander-Jansson,A;Dobec,M;Price,P;Wunderli,W;Rymo,L
利用杆状病毒表达系统产生全长EB病毒核抗原(EBNAs)2A和2B。构建了含有EBNA-2A和EBNA-2B编码序列的重组杆状病毒。用重组病毒感染果蝇SF-9细胞，用免疫印迹和免疫荧光技术对重组蛋白进行了鉴定。用部分纯化的EBNA-2A和EBNA-2B感染的昆虫细胞提取液，建立了检测EBNA-2A和EBNA-2B抗体的新的酶联免疫吸附试验。36%的瑞士急性传染性单核细胞增多症患者和81%的潜在EB病毒感染患者的血清标本对A型或B型EBNA-2蛋白有优先反应。在后一组患者中，76%的患者血清优先与EBNA-2A反应，5%的患者血清优先与EBNA-2B反应，12%的患者血清与两种抗原反应相似，7%的患者血清无反应。
The baculovirus expression system was used to produce full-length Epstein-Barr virus nuclear antigens (EBNAs) 2A and 2B. Recombinant baculoviruses that contained the EBNA-2A- and EBNA-2B-encoding sequences were constructed. The proteins were expressed in Spodoptera frugiperda SF-9 cells infected with the recombinant viruses and were characterized by using monoclonal and human polyclonal antibodies by immunoblotting and immunofluorescence techniques. Partially purified extracts of the EBNA-2A- and EBNA-2B-infected insect cells were used to establish a new enzyme-linked immunosorbent assay for the detection of antibodies against EBNA-2A and EBNA-2B. Preferential reactivity toward the type A or type B EBNA-2 protein was observed in 36% of serum specimens from Swiss patients with acute infectious mononucleosis and in 81% of Swiss patients with latent Epstein-Barr virus infection. Of the patients in the latter group, sera from 76% reacted preferentially with EBNA-2A, sera from 5% reacted preferentially with EBNA-2B, sera from 12% showed similar reactivities against both antigens, and sera from 7% were nonreactive.