The effect of graded steatosis on flow in the hepatic parenchymal microcirculation
The effect of graded steatosis on flow in the hepatic parenchymal microcirculation
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DOI：
10.1097/00007890-199909270-00009
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发表时间：
1999-09-27
期刊：
影响因子：
6.2
通讯作者：
Davidson, BR
中科院分区：
文献类型：
--
作者：
Seifalian, AM;Piasecki, C;Davidson, BR
背景：脂肪变性是肝移植后微循环障碍和移植物功能障碍的主要原因。这种循环妥协的机制尚不清楚。本研究的目的是在动物模型上评价脂肪变性对肝实质微循环和肝脏总血流量的影响。实验分4组(n=24)新西兰大白兔。第1组按正常饮食喂养(对照组)。第2、3、4组分别喂饲高胆固醇饲料(1.5%)4、8、12周，造成大鼠不同程度的脂肪变性。剖腹暴露肝脏后，测量肝脏总血流量(THBF)和肝实质微循环(HPM)。这些参数与轻度(60%)的组织学脂肪渗透程度相关。4周、8周和12周的胆固醇饮食分别导致轻度、中度和重度脂肪变性，脂肪渗透程度与两种高密度脂蛋白呈负相关(Spearman r=-0.967，P
Background: Steatosis is a major cause of microcirculatory Impairment and graft dysfunction after liver transplantation. The mechanism of this circulatory compromise is unclear. The aim of this study was to evaluate in vivo the effect of steatosis on parenchymal microcirculation and on total hepatic blood flow in an animal model.Methods. Four groups of New Zealand White rabbits (n=24) were investigated. Group 1 were fed on normal diet (controls). In groups 2, 3, and 4 graded steatosis was induced by feeding on a high cholesterol diet (1.5%) for 4, 8, and 12 weeks, respectively. After laparotomy and exposure of the liver, total hepatic blood flow (THBF) and the hepatic parenchymal microcirculation (HPM) were measured. These parameters were correlated with the degree of histological fat infiltration classified as mild (60%).Results. The 4-, 8-, and 12-week cholesterol diets resulted in mild, moderate, and severe steatosis, respectively There was an inverse correlation between the degree of fat infiltration and both HPM (Spearman r=-0.967, P