Functional moiety for the antifungal activity of phytocassane E, a diterpene phytoalexin from rice
Functional moiety for the antifungal activity of phytocassane E, a diterpene phytoalexin from rice
复制标题
DOI：
10.1016/s0031-9422(96)00534-1
复制
发表时间：
1997-01-01
期刊：
影响因子：
3.8
通讯作者：
Shimura, M
中科院分区：
文献类型：
--
作者：
Koga, J;Ogawa, N;Shimura, M
用马铃薯致病真菌致病疫霉（Phytophthora infestans）的菌丝提取物处理悬浮培养的水稻细胞，产生大量的植物抗毒素。其中，一个新的卡桑型二萜，命名为植物卡桑E，被分离和鉴定为1 β-羟基-12，15-木薯二烯-3，11-二酮。植物卡桑E抑制稻瘟病菌孢子萌发和芽管生长的艾德（50）值分别为6和2 μ g/ml。这一结果与早期的结果一起表明，在C-1位的羟基基团是植物卡桑E的高抗真菌活性的主要功能部分。版权所有（C）1996 Elsevier Science Ltd
Large amounts of phytoalexins were produced in suspension-cultured rice cells by treatment with a mycelial extract of the potato pathogenic fungus Phytophthora infestans. Among them, a new cassane-type diterpene, designated phytocassane E, was isolated and identified as 1 beta-hydroxy-12,15-cassadien-3,11-dione. The ED(50) values of phytocassane E in the prevention of spore germination and germ tube growth of the rice pathogenic fungus Magnaporthe grisea were 6 and 2 mu g ml(-1), respectively. This result, together with earlier results, suggests that the hydroxyl group at the C-l position is the main functional moiety for the high antifungal activity of phytocassane E. Copyright (C) 1996 Elsevier Science Ltd