Quantitative resistance to symptomless growth of Leptosphaeria maculans (phoma stem canker) in Brassica napus (oilseed rape)
Quantitative resistance to symptomless growth of Leptosphaeria maculans (phoma stem canker) in Brassica napus (oilseed rape)
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DOI：
10.1111/j.1365-3059.2008.01957.x
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发表时间：
2009-04-01
期刊：
影响因子：
2.7
通讯作者：
Fitt, B. D. L.
中科院分区：
文献类型：
--
作者：
Huang, Y. J.;Pirie, E. J.;Fitt, B. D. L.
以甘蓝型油菜（Brassica napus）为研究对象，分别选用具有数量抗性的Darmor和无数量抗性的Eurol品种，在田间和对照环境下进行了对黄斑细鳞绦虫的数量抗性研究。在田间试验中，对3个生长季节秋冬季的光斑病发病数量和次年夏季茎溃疡病的严重程度进行了评价。在秋冬季节，达尔莫和欧鲁在叶片损伤数上无显著差异。然而，第二年夏天收获时，达莫的茎溃疡病没有欧鲁严重。在控制环境实验中，以子囊孢子为接种物，研究了不同温度（5-25℃）和不同湿润时间（12-72 h）下叶片损伤的发育情况；利用定量PCR对黄斑扁豆沿叶柄向茎方向无症状生长进行定量分析，并利用表达gfp的黄斑扁豆进行可视化观察；利用表达gfp的黄斑乳杆菌在茎组织内的生长情况进行了研究。达尔莫与欧尔在黄斑菌潜伏期上无显著差异，但达尔莫与欧尔在黄斑菌潜伏期上无显著差异。在黑斑落菌沿叶柄向茎生长的距离和叶柄中黑斑落菌DNA的数量上，黑斑落菌在黑斑落菌茎组织上的定殖范围小于黑斑落菌。结果表明，病原菌对甘蓝型油菜茎部的定殖过程中发生了对黄斑乳杆菌的定量抗性。
Quantitative resistance to Leptosphaeria maculans in Brassica napus was investigated in field and controlled environments using cultivars Darmor (with quantitative resistance) and Eurol (without quantitative resistance). In field experiments, numbers of phoma leaf spot lesions in autumn/winter and severity of stem canker the following summer were assessed in three growing seasons. There were no differences between Darmor and Eurol in number of leaf lesions in autumn/winter. However, stem cankers were less severe on Darmor than Eurol at harvest the following summer. In controlled-environment experiments, development of leaf lesions at different temperatures (5-25 degrees C) and wetness durations (12-72 h) was investigated using ascospore inoculum; symptomless growth of L. maculans along leaf petioles towards the stem was quantified using quantitative PCR and visualized using GFP-expressing L. maculans; growth of L. maculans within stem tissues was investigated using GFP-expressing L. maculans. There were more leaf lesions on Darmor than Eurol, although there was no difference between Darmor and Eurol in L. maculans incubation period. There were no differences between Darmor and Eurol in either distance grown by L. maculans along leaf petioles towards the stem or quantity of L. maculans DNA in leaf petioles, but L. maculans colonized stem tissues less extensively on Darmor than Eurol. It was concluded that quantitative resistance to L. maculans operates during colonization of B. napus stems by the pathogen.