Orthotopic and heterotopic tracheal transplantation model in studying obliterative bronchiolitis
Orthotopic and heterotopic tracheal transplantation model in studying obliterative bronchiolitis
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原位和异位气管移植模型在闭塞性细支气管炎研究中的应用
DOI：
10.1016/j.trim.2013.04.006
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发表时间：
2013-06-01
影响因子：
1.5
通讯作者：
Jiang, Ke
中科院分区：
文献类型：
--
作者：
Fan, Kai;Qiao, Xin-Wei;Jiang, Ke
背景：目前已有多种动物模型用于研究肺移植术后闭塞性细支气管炎的发病机制。在这项研究中，我们比较了三个流行的小鼠模型闭塞性细支气管炎的几个基本的病理变化，在一个相对较短的时间跨度transplant.Methods：每个受体小鼠同时接受原位，网膜内和皮下气管移植在同基因和异基因设置。未给予免疫抑制治疗。分别于移植后第14、21、28天取气管移植物进行组织学和免疫组化分析。来自不同移植部位的同基因气管移植物保留了正常的组织学结构，而其相应的同种异体移植物表现出更多的气道腔闭塞以及更多的CD 4（+）/CD 8（+）单核细胞和肌成纤维细胞浸润，但在所示时间段上皮再生和新生血管减少（P < 0.05）。与两种异位同种异体移植物相比，原位同种异体移植物对气管腔的阻塞较少，CD 4（+）/CD 8（+）单核细胞和肌成纤维细胞浸润较少，但再生上皮和新生血管较多（P < 0.05）。小鼠原位气管移植可作为研究闭塞性细支气管炎早期阶段的模型，异位气管移植可作为晚期闭塞性细支气管炎的模型。(C)2013作者Elsevier B. V.出版，保留所有权利。
Background: Several animal models have been established to investigate the mechanisms of obliterative bronchiolitis after lung transplantation. In this study, we compared three prevalent murine models of obliterative bronchiolitis in terms of several basic pathologic changes in a relatively short span of time after transplantation.Methods: Each of the recipient mice simultaneously received orthotopic, intra-omental and subcutaneous tracheal transplantation in both syngeneic and allogeneic settings. No immunosuppressive treatment was administered. Tracheal grafts were harvested on Day 14, 21 and 28 after transplantation for histological and immunohistochemical analyses.Results: Syngeneic tracheal grafts from different transplant sites retained normal histologic structures, while their corresponding allografts demonstrated more occlusion of the airway lumen as well as more infiltration of CD4(+)/CD8(+) mononuclear cells and myofibroblasts, but less regenerative epithelium and neovascularized vessels at indicated times (P < 0.05). Compared with two heterotopic allografts, orthotopic allografts had less occlusion of the tracheal lumen as well as less infiltration of CD4(+)/CD8(+) mononuclear cells and myofibroblasts, but more regenerative epithelium and neovascularized vessels (P < 0.05).Conclusions: Orthotopic tracheal transplantation in mice can be considered as a model to study early stages of obliterative bronchiolitis, and heterotopic tracheal transplantation can be a model for late stages of obliterative bronchiolitis. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.