Antibody-functionalized nano test tubes target breast cancer cells
Antibody-functionalized nano test tubes target breast cancer cells
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DOI：
10.2217/17435889.3.3.283
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发表时间：
2008-06-01
期刊：
影响因子：
5.5
通讯作者：
Martin, Charles R.
中科院分区：
文献类型：
--
作者：
Buyukserin, Fatih;Medley, Colin D.;Martin, Charles R.
目的：开发将生物医学有效载荷传递到特定细胞类型的纳米试管。方法：采用模板合成法制备二氧化硅纳米试管。将对乳腺癌细胞具有特异性的抗体附着到外管表面。将荧光团附着到纳米试管的内表面。将管与乳腺癌细胞一起孵育，并通过荧光显微镜研究与细胞表面的附着程度。结果如下：相对于用物种和同种型匹配的对照抗体在其外表面上修饰的管，用靶抗体在其外表面上修饰的管显示出对乳腺癌细胞的增强的附着。结论：这项工作是证明纳米试管可用作细胞特异性运载工具的第一步。
Aim: To develop nano test tubes that will deliver a biomedical payload to a specific cell type. Methods: The template-synthesis method was used to prepare silica nano test tubes. An antibody that is specific for breast cancer cells was attached to the outer tube surfaces. A fluorophore was attached to the inner surfaces of the nano test tubes. The tubes were incubated with the breast cancer cells and the extent of attachment to the cell surfaces was investigated by fluorescence microscopy. Results: Tubes modified on their outer surfaces with the target antibody showed enhanced attachment to breast-cancer cells, relative to tubes modified on their outer surfaces with a species and isotype-matched control antibody. Conclusions: This work is a first step toward demonstrating that nano test tubes can be used as cell-specific delivery vehicles.