The nucleotide sequence of the essential cell-division gene ftsZ of Escherichia coli.
The nucleotide sequence of the essential cell-division gene ftsZ of Escherichia coli.
复制标题
大肠杆菌细胞分裂必需基因 ftsZ 的核苷酸序列。
DOI：
10.1016/0378-1119(85)90179-9
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发表时间：
1985
期刊：
影响因子：
3.5
通讯作者：
Lutkenhaus,J
中科院分区：
文献类型：
--
作者：
Yi,QM;Lutkenhaus,J
报道了含有必需细胞分裂基因的大肠杆菌 DNA 的 1.8 kb 片段的核苷酸序列。 FtsZ 蛋白的 Mrof 为 40294，具有 23% 带电残基，计算等电点为 4.9。 theftsZgene 的密码子使用反映了高表达基因的密码子使用。同样位于该 DNA 片段上的是 theftsA 基因的 3' 末端和 theenvA 基因的 5' 末端。这些名称通过在这些基因末端定位 Tn5 插入来证实，这些插入使这些基因中的每一个都失活。 forftsZ 的潜在启动子与 theftsA 基因的 3' 端重叠。 Tn5 插入位于 theftsA 的 3' 末端以及该潜在启动子内。 ftsA 和 ftsZ 之间或 ftsZandenvA 之间没有明显的转录终止子。
The nucleotide sequence of a 1.8-kb fragment ofEscherichia coliDNA containing the essential cell division geneftsZis reported. The FtsZ protein has anMrof 40294 and has 23% charged residues with a calculated isoelectric point of 4.9. The codon usage of theftsZgene reflects that of a highly expressed gene. Also located on this DNA fragment is the 3' end of theftsAgene and the 5' end of theenvAgene. These designations were confirmed by locating Tn5insertions within the ends of these genes that inactivate each of these genes. A potential promoter forftsZoverlapped the 3' end of theftsAgene. A Tn5insertion was located within the 3' end of theftsAand within this potential promoter. No transcription terminators were evident betweenftsAandftsZor betweenftsZandenvA.