Effect of different dietary zinc sources on seminal plasma enzymatic activity, antioxidant, and immune-related gene expression in rainbow trout (Oncorhynchus mykiss)
Effect of different dietary zinc sources on seminal plasma enzymatic activity, antioxidant, and immune-related gene expression in rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1007/s10499-021-00778-3
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发表时间：
2021-09-13
影响因子：
2.9
通讯作者：
Eslamloo, Khalil
中科院分区：
文献类型：
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作者：
Kazemi, Esmaeil;Nazari, Sajad;Eslamloo, Khalil
研究不同锌源对虹鳟鱼血清酶活性、抗氧化相关基因表达及免疫功能的影响。雄性虹鳟鱼被喂食16周，饲料中补充有40 mg kg（-1）的矿物锌补充剂（MZ; ZnSO 4中心点7 H（2）O），40 mg kg（-1）的有机锌（OZ），40 mg kg（-1）的锌纳米颗粒（NZ）和对照饲料（CD）。16周后，与对照组相比，不同饲料组的鱼血清酶活性显著升高（P < 0.05）。精浆中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、乳酸脱氢酶（LDH）和超氧化物歧化酶（SOD）的含量以MZ组最低（P < 0.05）。结果表明，日粮中不同锌源均能上调铜锌超氧化物歧化酶基因的表达（Cu/Zn-SOD）、锰超氧化物歧化酶（Mn-SOD）和过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S-转移酶mu（GST）和核因子红细胞2相关因子2（Nrf 2）的基因表达，而谷胱甘肽S-转移酶（GST）的基因表达不被MZ和OZ显著改变。与NZ和CD组相比，MZ和OZ组显著上调头肾组织中TNF-α的转录（P < 0.05）。与CD组相比，OZ组和NZ组头肾IL-1 β mRNA水平无明显变化（P > 0.05）。结果还表明，细胞因子基因表达，特别是TNF-α和Lyz II，可能会影响鱼类繁殖。结果表明，与对照组相比，添加无机锌和有机锌的饲料能显著提高鱼的抗氧化能力和免疫功能（P < 0.05）。因此，建议在虹鳟鱼饲料中添加矿物质锌和有机锌，可有效提高虹鳟鱼的免疫系统和抗氧化能力。
The objective of the present work was to survey the effects of different sources of zinc supplements on serum enzymatic activities, the expression of genes related to antioxidant and immunity in rainbow trout (Oncorhynchus mykiss). Male rainbow trout were fed for 16 weeks on a diet supplemented with 40 mg kg(-1) of mineral zinc supplement (MZ; ZnSO4 center dot 7H(2)O), 40 mg kg(-1) of organic zinc (OZ), 40 mg kg(-1) of zinc nanoparticles (NZ), and control diet (CD). After 16 weeks, fish fed different diet revealed significant serum enzymatic activities compared to the control treatment (P < 0.05). The lowest levels of aspartate aminotransferase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), and superoxide dismutase (SOD) enzymes were observed in seminal plasma of fish in treatment fed on MZ (P < 0.05). Based on the results, dietary different zinc sources upregulated the gene expressions of Cu/Zn-superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD) and catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase mu (GST), and nuclear factor erythroid 2-related factor 2 (Nrf2), while the gene expression of glutathione S-transferase (GST) was not significantly modified by MZ and OZ. The MZ and OZ groups significantly (P < 0.05) upregulated the TNF-alpha transcription in the head kidney tissues when compared with the NZ and CD groups. Meanwhile, the OZ and NZ groups did not change IL-1 beta mRNA level of the head kidney when compared with the CD group (P > 0.05). The results also indicated that cytokine gene expression, especially TNF-alpha and Lyz II, may affect fish reproduction. The results revealed that fish fed diet containing mineral zinc and organic zinc supplement showed significantly higher antioxidant and immune response as compared to the control groups (P < 0.05). It is therefore recommended that dietary mineral zinc and organic zinc administration may effectively promote the immune system and antioxidant capacity of rainbow trout.