Preliminary validation of ERBB2 expression regulated by miR-548d-3p and miR-559
Preliminary validation of ERBB2 expression regulated by miR-548d-3p and miR-559
复制标题
miR-548d-3p和miR-559调控ERBB2表达的初步验证。
DOI：
10.1016/j.bbrc.2009.05.113
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发表时间：
2009-08-07
影响因子：
3.1
通讯作者：
Chen, Zheng-tang
中科院分区：
文献类型：
--
作者：
Chen, Hong;Sun, Jian-guo;Chen, Zheng-tang
ERBB 2过表达发生在许多类型的原发性人类肿瘤中，并且microRNA（miRNA）表达的改变与人类癌症中肿瘤发生的肿瘤抑制相关，然而，对作用于ERBB 2的天然miRNA知之甚少。在本研究中，ERBB 2的3 '-UTR的生物信息学分析揭示了miR-548 d-3 p和miR-559的靶元件。此外，预测的miRNA/mRNA相互作用实验验证表明，miR-548 d-3 p和miR-559都可以相互作用：特别是与ERBB 2 mRNA的3 '-UTR相互作用。miR-548 d-3 p与miR-559联合转染对ERBB 2 mRNA表达的抑制作用与miR-548 d-3 p或miR-559单独转染的作用不同。这些结果不仅支持了不同miRNA可以同时协同抑制特定靶mRNA的观点，而且初步验证了miR-548 d-3 p和miR-559在ERBB 2表达调控中的作用。这些数据为miRNAs在ERBB 2靶向治疗中的应用提供了分子基础。(c)2009年由Elsevier Inc.出版
ERBB2 overexpression occurs in numerous types of primary human tumors and alterations in microRNA (miRNA) expression have been associated with tumor suppression OF tumorigenesis in human cancer, nevertheless, little is known about natural miRNAs acting on ERBB2. In this study, bioinformatical analysis of the 3'-UTRs of ERBB2 revealed the target elernents for miR-548d-3p and miR-559. Moreover, a predicted miRNA/mRNA interaction experimental validation showed that both miR-548d-3p and miR-559 can interact: specifically with the 3'-UTR of the ERBB2 mRNA. And miR-548d-3p plus miR-559 transfection showed a cooperative regulation of translationally repressing ERBB2 mRNA rather than by either miR-548d-3p or miR-559 alone. These results not only Support the idea that different miRNAs can simultaneously and cooperatively repress a given target mRNA but also preliminarily validate the role of miR-548d-3p and miR-559 in regulating the ERBB2 expression. These data provide molecular basis for the application of miRNAs in ERBB2-targeted therapy. (c) 2009 Published by Elsevier Inc.