Experimental evolution makes microbes more cooperative with their local host genotype
Experimental evolution makes microbes more cooperative with their local host genotype
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DOI：
10.1126/science.abb7222
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发表时间：
2020-10-23
期刊：
影响因子：
56.9
通讯作者：
Frederickson, Megan E.
中科院分区：
文献类型：
--
作者：
Batstone, Rebecca T.;O'Brien, Anna M.;Frederickson, Megan E.
微生物组科学的进步需要更好地了解有益微生物如何适应宿主。我们测试了主机是否选择更合作的微生物菌株长达一年的进化实验和交叉接种实验，旨在探索固氮菌（根瘤菌）如何适应豆科植物。我们将细菌Ensifer meliloti与五种苜蓿基因型中的一种配对，这些基因型在“选择”细菌共生体的程度上各不相同。不受寄主选择的影响，E.苜蓿草很快适应了当地的宿主基因型，当它们与宿主共享进化历史时，衍生的微生物更有益。这种局部适应主要限于共生质粒，在假定的信号基因突变。因此，合作依赖于伴侣基因型之间的匹配，并随着细菌适应当地宿主而增加。
Advances in microbiome science require a better understanding of how beneficial microbes adapt to hosts. We tested whether hosts select for more-cooperative microbial strains with a year-long evolution experiment and a cross-inoculation experiment designed to explore how nitrogen-fixing bacteria (rhizobia) adapt to legumes. We paired the bacterium Ensifer meliloti with one of five Medicago truncatula genotypes that vary in how strongly they "choose" bacterial symbionts. Independent of host choice, E. meliloti rapidly adapted to its local host genotype, and derived microbes were more beneficial when they shared evolutionary history with their host. This local adaptation was mostly limited to the symbiosis plasmids, with mutations in putative signaling genes. Thus, cooperation depends on thematch between partner genotypes and increases as bacteria adapt to their local host.