CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells.
CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells.
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CD127表达与FOXP3和人类CD4+ T Reg细胞的抑制功能成反比。
DOI：
10.1084/jem.20060772
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发表时间：
2006-07-10
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调节性T（T reg）细胞是免疫耐受的关键调节因子。大多数T reg细胞是基于CD 4、CD 25和转录因子FoxP 3的表达来定义的。然而，事实证明，这些标记物对于唯一定义人类的这种专门T细胞亚群存在问题。我们发现IL-7受体（CD 127）在外周血中的CD 4 + T细胞亚群上下调。我们证明这些细胞中的大多数是FoxP 3+，包括那些表达低水平或不表达CD 25的细胞。CD 4、CD 25和CD 127的组合导致高度纯化的T reg细胞群，其占先前基于其他细胞表面标志物鉴定的显著更多的细胞。这些细胞在功能抑制测定中具有高度抑制性。事实上，仅基于CD 4和CD 127表达分离的细胞是无反应性的，尽管代表至少三倍的细胞数量（包括CD 25 + CD 4+和CD 25 − CD 4 + T细胞亚群），但与“经典”CD 4 + CD 25 hi T reg细胞亚群一样具有抑制性。最后，我们表明CD 127可用于定量1型糖尿病患者的T reg细胞亚群，支持使用CD 127作为人类T reg细胞的生物标志物。
Regulatory T (T reg) cells are critical regulators of immune tolerance. Most T reg cells are defined based on expression of CD4, CD25, and the transcription factor, FoxP3. However, these markers have proven problematic for uniquely defining this specialized T cell subset in humans. We found that the IL-7 receptor (CD127) is down-regulated on a subset of CD4+ T cells in peripheral blood. We demonstrate that the majority of these cells are FoxP3+, including those that express low levels or no CD25. A combination of CD4, CD25, and CD127 resulted in a highly purified population of T reg cells accounting for significantly more cells that previously identified based on other cell surface markers. These cells were highly suppressive in functional suppressor assays. In fact, cells separated based solely on CD4 and CD127 expression were anergic and, although representing at least three times the number of cells (including both CD25+CD4+ and CD25−CD4+ T cell subsets), were as suppressive as the “classic” CD4+CD25hi T reg cell subset. Finally, we show that CD127 can be used to quantitate T reg cell subsets in individuals with type 1 diabetes supporting the use of CD127 as a biomarker for human T reg cells.