Labeling of neural cells by gold-filled Sendai virus envelopes before intracerebral transplantation.
Labeling of neural cells by gold-filled Sendai virus envelopes before intracerebral transplantation.
复制标题
脑内移植前用装金仙台病毒包膜标记神经细胞。
DOI：
10.1126/science.2829355
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Arendash,GW
中科院分区：
文献类型：
--
作者：
Ardizzoni,SC;Michaels,A;Arendash,GW
将充满金的仙台病毒包膜与来自胎鼠供体基底前脑的细胞悬浮液融合，并将所得的金标记细胞移植到成年大鼠受体的新皮质中。不仅大量的标记细胞在新皮层中保持完整3个月，而且相当数量的标记细胞在皮层下迁移到受体受损的基底核区域（4至5毫米的距离）。由于这种技术能够长时间标记大多数移植细胞，因此它可能有助于确定脑内移植组织的存活，迁移和连接。
Gold-filled Sendai virus envelopes were fused with cell suspensions from the basal forebrain of fetal rat donors, and the resulting gold-labeled cells were transplanted into the neocortex of adult rat recipients. Not only did large numbers of labeled cells remain intact through 3 months in the neocortex, but sizable numbers migrated subcortically to the recipient's lesioned nucleus basalis region (a distance of 4 to 5 millimeters). Since this technique is capable of labeling most transplanted cells for long periods of time, it may be useful in determining the survival, migration, and connectivity of intracerebrally transplanted tissues.