Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States
Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States
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DOI：
10.1099/0022-1317-82-10-2449
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发表时间：
2001-10-01
影响因子：
3.8
通讯作者：
Meng, XJ
中科院分区：
文献类型：
--
作者：
Haqshenas, G;Shivaprasad, HL;Meng, XJ
肝炎-脾肿大综合征(HS)是北美鸡的一种新出现的疾病，其病因尚不清楚。本文报道了从HS综合征鸡胆汁中分离到的一株与人类戊型肝炎病毒(HEV)相关的新病毒的基因鉴定和特性。基于该病毒与HEV相似的基因组结构和显著的序列同源性，为了区别于人和猪的HEV，将其暂定为Avive HEV。电子显微镜观察表明，禽HEV是一种直径30-35 nm的无包膜病毒颗粒。测定了病毒基因组3‘端的序列(与4kb相似)。序列分析表明，该基因包含完整的3‘非编码区、开放阅读框(ORF)2和3的完整基因、依赖于RNA的RNA聚合酶(RdRp)基因和ORF 1的部分解旋酶基因。解旋酶基因是禽HEV与其他HEV毒株之间最保守的基因，其序列同源性分别为58-61%和57-60%。禽HEV的RdRp基因与其他已知HEV毒株的相应区域有47%-50%的AS和52%-53%的核苷酸序列同源性，而可能的衣壳基因(ORF2)与其他已知HEV毒株的相应区域有48%-49%的AS和4851%的NT序列同源性。系统发育分析表明，禽类HEV在基因上与其他已知HEV毒株有亲缘关系，但不同于其他已知HEV毒株。这一发现对HEV动物模型、命名和自然历史具有重要意义。
Hepatitis-splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the genetic identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a nonenveloped virus particle of 30-35 nm in diameter. The sequence of the 3' half of the viral genome (similar to 4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3' non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58-61 % as and 57-60% nt sequence identities. The RdRp gene of avian HEV shares 47-50% as and 52-53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48-49% as and 48-51 % nt sequence identities with the corresponding regions of other known HEV strains. Phylogenetic analysis indicates that avian HEV is genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.