CYTOTOXIC INTERACTIVE EFFECTS OF DENTIN BONDING COMPONENTS ON MOUSE FIBROBLASTS
CYTOTOXIC INTERACTIVE EFFECTS OF DENTIN BONDING COMPONENTS ON MOUSE FIBROBLASTS
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DOI：
10.1177/00220345950740091601
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发表时间：
1995-09-01
影响因子：
7.6
通讯作者：
DENNISON, JB
中科院分区：
文献类型：
--
作者：
RATANASATHIEN, S;WATAHA, JC;DENNISON, JB
先前的研究表明，牙髓对牙本质粘接系统有多种反应，并且牙本质粘接剂的体外和体内毒性之间的相关性较差。由于牙本质粘结剂由多种成分组成，这些成分可以通过牙本质扩散，因此我们假设这些成分可能通过相互作用（协同）效应引起细胞毒性。我们研究了四种牙本质粘合成分（HEMA、Bis-GMA、TEGDMA 和 UDMA）的细胞毒性，以及牙本质粘合成分的三种二元组合（HEMA 和 Bis-GMA、Bis-GMA 和 TEGDMA、TEGDMA 和 UDMA）的相互作用效应。通过 MTT 测定测量对 Balb/c 3T3 小鼠成纤维细胞的细胞毒性。与对照相比，引起 50% 毒性的浓度（TC50 值）进行了比较，并通过评估观察到的细胞 MTT 活性与预期的 MTT 活性之间的差异来确定交互作用。根据 TC50 值，牙本质粘合成分的毒性排名如下：Bis-GMA > UDMA > TEGDMA >>> HEMA（毒性最低），暴露 24 小时和 72 小时后。作为二元组合，牙本质粘合成分的三种组合以三种方式相互作用——协同作用、加成作用和拮抗作用——这受到两种成分浓度的影响。较长的暴露时间导致牙本质粘合成分和组合的细胞毒性显着增加。研究结果表明，暴露时间和牙本质粘合成分之间的相互作用可能是确定牙本质粘合剂体内细胞毒性的重要参数。
Previous studies have shown a wide range of pulpal reactions to dentin bonding systems and a poor correlation between in vitro and in vivo toxicity of dentin bonding agents. Because dentin bonding agents are composed of multiple components which may diffuse through dentin, we hypothesized that these components may cause cytotoxicity through interactive (synergistic) effects. We investigated the cytotoxicities of four dentin bonding components-HEMA, Bis-GMA, TEGDMA, and UDMA--and interactive effects for three binary combinations of the dentin bonding components-HEMA and Bis-GMA, Bis-GMA and TEGDMA, and TEGDMA and UDMA. Cytotoxicities to Balb/c 3T3 mouse fibroblasts were measured by the MTT assay. Concentrations which caused 50% toxicity compared with controls (TC50 values) were compared, and the interactive effects were determined by evaluation of the differences between observed and expected MTT activities of the cells. The ranks of toxicity of the dentin bonding components in terms of TC50 values were as follows: Bis-GMA > UDMA > TEGDMA >>> HEMA (least toxic) after 24- and 72-hour exposures. As binary combinations, the three combinations of dentin bonding components interacted in three ways-synergism, additivism, and antagonism-which were influenced by the concentrations of both components. The longer period of exposure resulted in a significant increase in the cytotoxicity of the dentin bonding components and combinations. The findings indicate that both exposure time and the interactions between the dentin bonding components may be important parameters in determining the cytotoxicity of dentin bonding agents in vivo.