Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation
Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation
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DOI：
10.1177/0271678x20928011
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发表时间：
2020-06-09
影响因子：
6.3
通讯作者：
Sakadzic, Sava
中科院分区：
文献类型：
--
作者：
Sencan, Ikbal;Esipova, Tatiana;Sakadzic, Sava
大脑皮层具有许多跨脑区域和物种的保守的形态和功能特征。其中，微血管密度和线粒体细胞色素c氧化酶染色的层差异表明基线O（2）代谢的潜在层变异性和/或O（2）需求和血流动力学反应的层变异性。在这里，我们调查的刺激诱导的血管内分压的O-2（pO 2）瞬变刺激诱导的神经元激活在完全清醒的小鼠使用双光子磷光寿命显微镜的层流配置文件。我们的研究结果表明，刺激诱导的血管内pO（2）的变化在皮层I-IV层是保守的，这表明一个严格控制的神经血管反应，以提供足够的O（2）供应整个皮层深度。此外，我们观察到与小动脉相比，小静脉O（2）饱和度（Δ sO（2））的变化更大，小静脉对皮质表面的Δ sO（2）反应逐渐增加，并且没有先前报告的麻醉下血管内“初始下降”。这项研究为定量特定层脑O（2）代谢反应铺平了道路，促进了健康和疾病中脑能量学的研究，并为解释层血氧水平依赖的功能磁共振成像信号提供了信息。
The cerebral cortex has a number of conserved morphological and functional characteristics across brain regions and species. Among them, the laminar differences in microvascular density and mitochondrial cytochrome c oxidase staining suggest potential laminar variability in the baseline O(2)metabolism and/or laminar variability in both O(2)demand and hemodynamic response. Here, we investigate the laminar profile of stimulus-induced intravascular partial pressure of O-2(pO2) transients to stimulus-induced neuronal activation in fully awake mice using two-photon phosphorescence lifetime microscopy. Our results demonstrate that stimulus-induced changes in intravascular pO(2)are conserved across cortical layers I-IV, suggesting a tightly controlled neurovascular response to provide adequate O(2)supply across cortical depth. In addition, we observed a larger change in venular O(2)saturation (Delta sO(2)) compared to arterioles, a gradual increase in venular Delta sO(2)response towards the cortical surface, and absence of the intravascular "initial dip" previously reported under anesthesia. This study paves the way for quantification of layer-specific cerebral O(2)metabolic responses, facilitating investigation of brain energetics in health and disease and informed interpretation of laminar blood oxygen level dependent functional magnetic resonance imaging signals.