Chitosan-RGDSGGC conjugate as a scaffold material for musculoskeletal tissue engineering.
Chitosan-RGDSGGC conjugate as a scaffold material for musculoskeletal tissue engineering.
复制标题
DOI：
10.1016/j.biomaterials.2005.01.062
复制
发表时间：
2005-09
期刊：
影响因子：
14
通讯作者：
T. Masuko;N. Iwasaki;S. Yamane;T. Funakoshi;T. Majima;A. Minami;Noriko Ohsuga;T. Ohta;S. Nishim
中科院分区：
文献类型：
--
作者：
T. Masuko;N. Iwasaki;S. Yamane;T. Funakoshi;T. Majima;A. Minami;Noriko Ohsuga;T. Ohta;S. Nishim
本研究基于壳聚糖与2-亚氨基硫杂环戊烷的选择性反应，发展了一种制备壳聚糖-肽复合物的新方法。新型SH-壳聚糖衍生物在碱性条件下也表现出良好的水溶性。这一特性极大地促进了壳聚糖与多种生物活性物质的进一步改性研究。合成的含有RGDS部分的合成肽RGDSGGC被认为是最重要的细胞粘附肽之一，在二甲基亚砜存在下，其容易通过二硫键形成与SH-壳聚糖的巯基偶联。接下来，评价了将RGDSGGC部分引入壳聚糖对软骨细胞和成纤维细胞的细胞粘附和增殖活性的影响。结果表明，该多糖-肽缀合物对软骨细胞和成纤维细胞的细胞粘附和细胞增殖都表现出优异的能力。考虑到生物相容性支架在最近定制的组织工程技术中的日益重要性，这些结果表明，目前的策略，2-亚氨基硫杂环戊烷为基础的多糖与生物活性肽的缀合将成为一个关键和潜在的技术，开发理想的支架材料的组织再生。
In the present study, we have developed a novel and versatile method for the preparation of chitosan-peptide complex based on the selective reaction of chitosan with 2-iminothiolane. The new type of SH-chitosan derivative showed an excellent solubility to aqueous solution even in the alkaline conditions. This characteristic greatly facilitated further modification study of chitosan with a variety of bioactive substances. A synthetic peptide, RGDSGGC containing RGDS moiety that is known as one of the most important cell adhesive peptides, was readily coupled by disulfide bonds formation with sulfhydryl groups of SH-chitosan in the presence of dimethyl sulfoxide. Next, the effect of the introduction of RGDSGGC moiety to chitosan on cell adhesion and proliferation activity of chondrocytes and fibroblasts were evaluated. As a result, it was suggested that this polysaccharide-peptide conjugate exhibited excellent capacities for both cell adhesion and cell proliferation of chondrocytes and fibroblasts. Considering the growing importance of the biocompatible scaffolds in the recent tailored tissue engineering technique, these results indicate that the present strategy of 2-iminothiolane-based conjugation of polysaccharides with biologically active peptides will become a key and potential technology to develop desirable scaffold materials for the tissue regenerations.