Partial characterization of the molecular nature of collagen-linked fluorescence: Role of diabetes and end-stage renal disease
Partial characterization of the molecular nature of collagen-linked fluorescence: Role of diabetes and end-stage renal disease
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DOI：
10.1016/j.abb.2009.10.013
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发表时间：
2010-01-15
影响因子：
3.9
通讯作者：
Monnier, Vincent M.
中科院分区：
文献类型：
--
作者：
Sell, David R.;Nemet, Ina;Monnier, Vincent M.
在激发/发射370/440 nm处的胶原蛋白相关荧光已被广泛用作衰老、糖尿病并发症和终末期肾病（ESRD）研究中晚期糖基化的标志物。在此波长下测量皮肤自发荧光的诊断设备揭示了荧光与心血管发病率和死亡率之间的关联。我们现在报告了人类皮肤胶原蛋白中存在一种主要荧光团（LW-1），该荧光团随着年龄、糖尿病和ESRD的增加而增加。其分子量为623.2 Da，UV最大值为348 nm，并在芳环中包含赖氨酸残基。LW-1不能使用传统的糖基化化学合成，这表明一种复杂的形成机制，可能与缺氧有关，因为在患有慢性肺病的非糖尿病个体中也发现了升高的水平。(C)2009 Elsevier Inc. All rights reserved.
Collagen-linked fluorescence at excitation/emission 370/440 nm has widely been used as a marker for advanced glycation in studies of aging, diabetic complications, and end-stage renal disease (ESRD). Diagnostic devices measuring skin autofluorescence at this wavelength revealed an association between fluorescence and cardiovascular morbidity and mortality. We now report the presence of a major fluorophore (LW-1) in human skin collagen which increases with age, diabetes, and ESRD. It has a molecular weight of 623.2 Da, a UV maximum at 348 nm, and involves a lysine residue in an aromatic ring. LW-1 could not be synthesized using traditional glycation chemistry suggesting a complex mechanism of formation, perhaps related to hypoxia since elevated levels were also found in nondiabetic individuals with chronic lung disease. (C) 2009 Elsevier Inc. All rights reserved.