Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.
Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.
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DOI：
10.3389/fpls.2016.00511
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发表时间：
2016
影响因子：
5.6
通讯作者：
Zheng S
中科院分区：
文献类型：
--
作者：
Liu M;Xu J;Lou H;Fan W;Yang J;Zheng S
水稻豆（VuMATE1） VuMATE1主要在维管系统而非根端表达，铝胁迫将其表达扩展到根端。VuMATE1是否同时参与Al耐受性和Fe营养，以及VuMATE1的表达是如何被调控的，是人们非常感兴趣的。在本研究中，VuMATE1在铁营养中的作用通过植物内补体实验进行了表征。通过启动子分析和启动子- gus报告子分析来研究VuMATE1的转录调控。结果表明，VuMATE1的表达受Al胁迫的调控，而不受Fe状态的调控。VuMATE1启动子下frd3-1与VuMATE1的互补不能恢复表型，但在35SCaMV启动子下恢复表型。VuMATE1免疫染色显示VuMATE1在血管中的异常定位。在植物GUS报告基因分析中，鉴定出al响应的顺式作用元件位于-1228 ~ -574 bp之间。启动子分析揭示了几个顺式作用元件，但转录不是简单地由这些元件之一调节的。我们发现VuMATE1表达的顺式调控参与了Al耐受机制，而不参与Fe营养机制。这些结果揭示了VuMATE1基因表达的进化有利于水稻豆更好地适应铝胁迫而铁缺乏压力释放的酸性土壤。
Rice bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acid soils where Al stress imposed but Fe deficiency pressure released.