Tanshinone IIA downregulates the CD40 expression and decreases MMP-2 activity on atherosclerosis induced by high fatty diet in rabbit
Tanshinone IIA downregulates the CD40 expression and decreases MMP-2 activity on atherosclerosis induced by high fatty diet in rabbit
复制标题
丹参酮IIA下调CD40表达并降低MMP-2活性对高脂饮食诱导的兔动脉粥样硬化
DOI：
10.1016/j.jep.2007.09.025
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发表时间：
2008-01-17
影响因子：
5.4
通讯作者：
Zhang, Hui
中科院分区：
文献类型：
--
作者：
Fang, Zhi-Yuan;Lin, Rong;Zhang, Hui
丹参酮IIA（Tanshinone IIA）是从丹参根中提取的主要脂溶性成分之一，具有抗动脉粥样硬化的作用。为探讨其可能的作用机制，本研究采用高脂饲料喂养家兔建立动物模型，并给予不同剂量的丹参Ⅱ A。采用分光光度法检测血清中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量，Western Blotting法检测血清细胞膜组分中分化抗原CD 40（cluster of differentiation 40，CD 40）的表达，酶谱法检测血清总蛋白提取物中基质金属蛋白酶-2（MMP-2）的活性。与对照组比较，模型组MDA含量、CD 40表达及MMP-2活性均显著升高，SOD活性显著降低。丹参Ⅱ A组SOD活性明显升高，NIDA水平明显降低，CD 40表达和MMP-2活性均降低。提示Tan Ⅱ A不仅具有抗氧化作用，而且具有抑制动脉粥样硬化病变炎症反应的作用。提示Tan IIA抗动脉粥样硬化的作用机制可能与其抗氧化作用和降低CD 40表达及MMP-2活性有关。
Tanshinone IIA (Tan IIA) is a member of the major lipophilic components abstracted from the root of Salvia miltiorrhiza Bunge and has the capacity of anti-atherosclerosis. To investigate the potential mechanism, we established an animal model by giving high fatty diet to rabbits and Tan IIA was given in different dose. Then, superoxide dismutase (SOD) activity and the malondialdehyde (MDA) level in serum were detected using spectrophotometry; cluster of differentiation 40 (CD40) expression of cellular membrane fraction of aortas and matrix metalloproteinase-2 (MMP-2) activity of total protein extract of aortas were detected by Western Blotting and Zymography, respectively. Compared with the control group, the level of MDA, the expression of CD40 and the MMP-2 activity were increased while the SOD activity was decreased significantly in model group. After Tan IIA administration, the SOD activity was significantly increased while the level of NIDA was decreased; both the expression of CD40 and the activity of MMP-2 were decreased. It is suggested that Tan IIA not only inhibits the oxidation but also suppresses the inflammation in atherosclerotic lesion. Our data suggest that not only anti-oxidation but also anti-inflammation by decrease the expression of CD40 and MMP-2 activity maybe the potential mechanisms by which Tan IIA anti-atherosclerosis.