GRANZYME-POSITIVE CYTOTOXIC-CELLS ARE SPECIFICALLY INCREASED IN EARLY RHEUMATOID SYNOVIAL TISSUE
GRANZYME-POSITIVE CYTOTOXIC-CELLS ARE SPECIFICALLY INCREASED IN EARLY RHEUMATOID SYNOVIAL TISSUE
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DOI：
10.1002/art.1780371205
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发表时间：
1994-12-01
影响因子：
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通讯作者：
BREEDVELD, FC
中科院分区：
文献类型：
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作者：
TAK, PP;KUMMER, JA;BREEDVELD, FC
Objective.研究类风湿关节炎（RA）患者滑膜组织（ST）中颗粒酶（Gran）A和B阳性细胞毒性细胞的表达和表型，并与病程和活动性进行比较。使用抗GranA和GranB的抗体，作为活化的自然杀伤（NK）细胞和细胞毒性T淋巴细胞的标志物，ST段10例早期RA，10例长期RA，10例骨关节炎患者进行了检查。用双标记技术检测细胞表型。在所有组患者的ST中均发现了Gran+细胞，其中大部分是NK细胞。这些细胞中有几种不表达表面标志物CD16、CD56和CD57。ST段GranB表达在早期RA患者中最高。在RA患者中，GranB表达与血清急性期反应物水平呈正相关，但与炎症组织学评分无相关性。Gran+细胞主要是NK细胞，其中很大一部分不表达常规NK细胞表面标志物。GranB表达在短时间RA患者的滑膜组织中特异性增加。
Objective. To define the expression and the phenotype of granzyme (Gran) A and B positive cytotoxic cells in synovial tissue (ST) from patients with rheumatoid arthritis (RA) with respect to disease duration and activity.Methods. Using antibodies against GranA and GranB, which serve as markers of activated natural killer (NK) cells and cytotoxic T lymphocytes, ST sections from 10 patients with early RA, 10 patients with longstanding RA, and 10 patients with osteoarthritis were examined. The phenotype of Gran+ cells was determined with double-labeling techniques.Results. Gran+ cells, the majority of which-were NK cells, were found in ST from patients in all groups. Several of these cells did not express the surface markers CD16, CD56, and CD57. The highest ST expression of GranB was found in patients with early RA. In RA patients, there was a positive correlation of GranB expression with serum levels of acute-phase reactants, but not with histologic scores for inflammation.Conclusion. Gran+ cells are mainly NK cells, a substantial proportion of which do not express conventional NK cell surface markers. GranB expression is specifically increased in the synovial tissues of patients with RA of short duration.