Dmbx1, a novel evolutionarily conserved paired-like homeobox gene expressed in the brain of mouse embryos
Dmbx1, a novel evolutionarily conserved paired-like homeobox gene expressed in the brain of mouse embryos
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DOI：
10.1016/s0925-4773(01)00587-1
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发表时间：
2002-01-01
影响因子：
2.6
通讯作者：
Behringer, RR
中科院分区：
文献类型：
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作者：
Ohtoshi, A;Nishijima, I;Behringer, RR
为了确定在小鼠胚胎发生过程中表达的新的同源盒基因，我们搜索了数据库，发现了一个新的小鼠成对的同源盒基因Dmbx1(间脑/中脑表达的脑同源盒基因1)，它在斑马鱼和人类中也是保守的。连锁分析将小鼠DMBX1定位于4号染色体的中间部分，这是人类1号染色体的同源基因簇区，人类DMBX1位于该区域。小鼠和人的DMBX1/DMBX1都有4个编码外显子，其基因结构是保守的。整装原位杂交显示Dmbx1在7.5-9.5DPC小鼠胚胎中有表达。在7.5和8.5dpc时，Dmbx1在前皱褶的一个亚区表达。在9.5dpc时，在尾侧间脑和中脑也观察到表达，并且仅限于神经上皮细胞。在成体组织中检测到脑、胃和睾丸中的表达。Dmbx1为发育中的前神经系统提供了一个独特的标记物，也为阐明脊椎动物大脑模式的机制提供了有用的分子资源。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
To identify novel homeobox genes expressed during mouse embryogenesis, we searched the databases and found a novel mouse paired-like homeobox gene, Dmbx1(diencephalon/mesencephalon-expressed brain homeobox gene 1), that is also conserved in zebrafish and human. Linkage analysis mapped mouse Dmbx1 to the mid-portion of chromosome 4 that is the homologous gene cluster region of human chromosome 1, where human DMBX1 is located. Both mouse and human Dmbx1/DMBX1 have four coding exons and their gene structures are conserved. Whole-mount in situ hybridization revealed that Dmbx1 expression is detected in 7.5-9.5 dpc mouse embryos. At 7.5 and 8.5 dpc, Dmbx1 is expressed in a sub-region of the anterior head folds. At 9.5 dpc, expression is observed in the caudal diencephalon as well as in the mesencephalon and is restricted to the neuroepithelium. Expression in adult tissues was detected in brain, stomach, and testis. Dmbx1 provides a unique marker of the developing anterior nervous system and should provide a useful molecular resource to elucidate the mechanisms that pattern the vertebrate brain. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.