Micro free-flow electrophoresis with injection molded chips
Micro free-flow electrophoresis with injection molded chips
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DOI：
10.1039/c1ra00874a
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发表时间：
2012-01-01
期刊：
影响因子：
3.9
通讯作者：
Belder, Detlev
中科院分区：
文献类型：
--
作者：
Koehler, Stefan;Benz, Christian;Belder, Detlev
在这项工作中，我们提出了一种经济的自由流动电泳芯片制造的注塑成型的大量复制过程。这是通过开发适合于一步成型制造的自由流动电泳芯片设计来实现的。集成的分隔条作为气泡分离的关键元件。在分离室和电极通道之间集成了一个高20 μ m、宽500 μ m的限定的开放间隙，作为气泡的屏障，同时通过流体接头保持电接触。此外，我们提出了一种方法，以避免在FFE微芯片的一个简单的大规模生产过程中的内部电极。注塑成型的热塑性mu FFE芯片可以随时使用，而无需随后的劳动密集型膜或用作作为盐桥的类似结构的实施。
In this work we present an approach towards economic free-flow electrophoresis chips fabricated by injection molding as mass replication process. This is achieved by the development of a free-flow electrophoresis chip design suitable for one step molding fabrication. Integrated partitioning bars are incorporated as key elements for bubble segregation. A defined open gap of 20 mu m in height and 500 mu m in width was integrated between the separation chamber and the electrode channels, acting as a barrier for gas bubbles while maintaining electrical contact by the fluidic junction. Additionally, we present an approach to avoid internal electrodes in FFE microchips for a facile mass production process. The injection molded thermoplastic mu FFE chips are ready to use without subsequent labor-intensive implementation of membranes or comparable structures serving as salt bridges.