Gastrodin decreases immunoreactivities of γ-aminobutyric acid shunt enzymes in the hippocampus of seizure-sensitive gerbils
Gastrodin decreases immunoreactivities of γ-aminobutyric acid shunt enzymes in the hippocampus of seizure-sensitive gerbils
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DOI：
10.1002/jnr.10502
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发表时间：
2003-02-15
影响因子：
4.2
通讯作者：
Kang, TC
中科院分区：
文献类型：
--
作者：
An, SJ;Park, SK;Kang, TC
天麻素是从天麻中分离出来的天然化合物之一，具有抗惊厥作用，尽管这种天然化合物的确切药理原理及其对体内γ -氨基丁酸（GABA）代谢的其他方面的影响尚未被探索。因此，本研究通过检测天麻素对沙鼠海马GABA代谢的影响，试图确定该物质的抗癫痫特性。天麻素降低了治疗组的癫痫发作评分，尽管在天麻素治疗的动物中，GABA合成酶和GABA转运体的免疫反应性没有改变。有趣的是，在天麻素处理组，海马，特别是神经元中GABA转氨酶（GABA- t）的免疫反应性显著降低。天麻素处理组海马组织中琥珀酸半醛脱氢酶（SSADH）和琥珀酸半醛还原酶（SSAR）的免疫反应活性也明显降低，与未处理组相比，未处理组海马组织中SSADH和SSAR的免疫反应活性较强。从神经解剖学角度看，天麻素可能通过抑制GABA分流引起GABA浓度升高。(C) 2002 Wiley-Liss, Inc。
Gastrodin is one of the natural compound isolated from Gastrodia elata and has known anticonvulsant effects, although the exact pharmacological principles of this natural compound and its effects on other aspects of gamma-aminobutyric acid (GABA) metabolism in vivo have not been explored. Therefore, in the present study, the effects of gastrodin on GABA metabolism in the gerbil hippocampus were examined, in an effort to identify the antiepileptic characteristics of this substance. Gastrodin reduced the seizure score in the treated group, although the immunoreactivities of GABA synthetic enzymes and GABA transporters were unaltered in gastrodin-treated animals. Interestingly, in the gastrodin-treated group, GABA transaminase (GABA-T) immunoreactivity in the hippocampus, particularly in neurons, was significantly decreased. In the gastrodin-treated group, both succinic semialdehyde dehydrogenase (SSADH) and succinic semialdehyde reductase (SSAR) immunoreactivities in the hippocampus was also decreased significantly, which stood in contrast to the nontreated group, in which strong SSADH and SSAR immunoreactivities were detected. From the neuroanatomical viewpoint, these findings suggest that gastrodin may cause the elevation of GABA concentration by inhibiting the GABA shunt. (C) 2002 Wiley-Liss, Inc.