Transcytosis of Murine-Adapted Bovine Spongiform Encephalopathy Agents in an In Vitro Bovine M Cell Model
Transcytosis of Murine-Adapted Bovine Spongiform Encephalopathy Agents in an In Vitro Bovine M Cell Model
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DOI：
10.1128/jvi.00969-10
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发表时间：
2010-09
影响因子：
5.4
通讯作者：
Kohtaro Miyazawa;T. Kanaya;Ikuro Takakura;Sachi Tanaka;Tetsuya Hondo;Hitoshi Watanabe;M. Rose;H. Kitazawa;Takahiro Yamaguchi;S. Katamine;N. Nishida;H. Aso
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作者：
Kohtaro Miyazawa;T. Kanaya;Ikuro Takakura;Sachi Tanaka;Tetsuya Hondo;Hitoshi Watanabe;M. Rose;H. Kitazawa;Takahiro Yamaguchi;S. Katamine;N. Nishida;H. Aso
摘要传染性海绵状脑病（TSE），包括牛海绵状脑病（BSE），是人类和动物的致命性神经退行性疾病。BSE似乎通过食用被BSE/羊瘙痒病因子污染的饲料传播给牛。在口腔感染的情况下，药物必须穿过肠上皮屏障。我们最近建立了一个牛小肠上皮细胞系（BIE细胞），该细胞系在体外经淋巴细胞刺激后可分化为M细胞类型（K。宫泽T. Hondo，T.卡纳亚河，巴西-地田中岛Takakura，W. Itani，M. T.罗斯，H。Kitazawa，T. Yamaguchi和H.阿索，组织化学。细胞生物学133：125-134，2010）。在这项研究中，我们评估了M细胞的作用，在肠道入侵的鼠适应性BSE（mBSE）代理使用我们的体外牛肠上皮模型。我们在此证明，在我们的体外模型中，M细胞分化的BIE细胞能够比未分化的BIE细胞更有效地运输mBSE试剂而不失活至少30倍。由于已知卵泡相关上皮中的M细胞具有将各种大分子、病毒和细菌从肠腔转运至粘膜免疫细胞的高能力，因此我们的结果表明牛M细胞能够递送TSE试剂而不仅仅是mBSE试剂的可能性。
ABSTRACT Transmissible spongiform encephalopathies (TSE), including bovine spongiform encephalopathy (BSE), are fatal neurodegenerative disorders in humans and animals. BSE appears to have spread to cattle through the consumption of feed contaminated with BSE/scrapie agents. In the case of an oral infection, the agents have to cross the gut-epithelial barrier. We recently established a bovine intestinal epithelial cell line (BIE cells) that can differentiate into the M cell type in vitro after lymphocytic stimulation (K. Miyazawa, T. Hondo, T. Kanaya, S. Tanaka, I. Takakura, W. Itani, M. T. Rose, H. Kitazawa, T. Yamaguchi, and H. Aso, Histochem. Cell Biol. 133:125-134, 2010). In this study, we evaluated the role of M cells in the intestinal invasion of the murine-adapted BSE (mBSE) agent using our in vitro bovine intestinal epithelial model. We demonstrate here that M cell-differentiated BIE cells are able to transport the mBSE agent without inactivation at least 30-fold more efficiently than undifferentiated BIE cells in our in vitro model. As M cells in the follicle-associated epithelium are known to have a high ability to transport a variety of macromolecules, viruses, and bacteria from gut lumen to mucosal immune cells, our results indicate the possibility that bovine M cells are able to deliver agents of TSE, not just the mBSE agent.