CBP1 function is required for stability of a hybrid cob-oli1 transcript in yeast mitochondria.
CBP1 function is required for stability of a hybrid cob-oli1 transcript in yeast mitochondria.
复制标题
CBP1 功能是酵母线粒体中杂交 cob-oli1 转录物稳定性所必需的。
DOI：
10.1007/bf00309911
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发表时间：
1990
期刊：
影响因子：
2.5
通讯作者：
Dieckmann,CL
中科院分区：
文献类型：
--
作者：
Mittelmeier,TM;Dieckmann,CL
核基因产物CBP1稳定酵母线粒体中的细胞色素b转录本。在突变株中，细胞色素基因(Cob)转录本在Northern印迹分析中未被检测到。以前的研究结果导致了这样的假设，即CBP1与COBmRNA的5‘-未翻译序列或Pre-mRNA相互作用，以稳定信息。为了确定该基因的哪一部分足以与CBP1相互作用，我们研究了一个新的杂交基因cob-oli1的转录本的稳定性，在该基因中，该基因的5‘端三分之一与ATP合成酶亚单位9基因的编码序列oli1融合在一起。杂合的cob-oli1转录本在cbp1基因座的野生型菌株中是陈旧的，但在cbp1突变背景中检测不到。线粒体蛋白的35S-蛋氨酸标记证实，在含有cob-oli1基因的CBP1菌株中，cob-oli1转录本翻译产生了ATP合成酶9亚单位。我们得出结论，CBP1的5‘末端部分足以发挥CBP1的功能，并讨论了CBP1直接与转录本的这一区域相互作用以促进转录稳定性的假设。
The nuclear gene product CBP1 stabilizes cytochromebtranscripts in yeast mitochondria. Incbp1 mutant strains, cytochromebgene (cob) transcripts are not detectable by Northern blot analysis. The results of previous studies led to the hypothesis that CBP1 interacts with the 5′-untranslated sequence of thecobmRNA, or pre-mRNA, to stabilize the message. To determine what portion of thecobleader is sufficient for interaction with CBP1, we have investigated the stability of transcripts from a novel hybrid gene,cob-oli1, in which the 5′-terminal third of thecobleader sequence was fused to the coding sequence of the gene for ATP synthase subunit 9,oli1. The hybridcob-oli1 transcript was stale in a strain wild-type at theCBP1 locus, but was undetectable in thecbp1 mutant background. That thecob-oli1 transcript was translated to produce ATP synthase subunit 9 inCBP1 strains containing thecob-oli1 gene was verified by35S-methionine labeling of mitochondrial proteins. We conclude that the 5′-terminal portion of thecobmessage is sufficient forCBP1 function and discuss the hypothesis that CBP1 interacts directly with this region of the transcript to promotecobmRNA stability.