200 Gy sterilised Toxoplasma gondii tachyzoites maintain metabolic functions and mammalian cell invasion, eliciting cellular immunity and cytokine response similar to natural infection in mice
200 Gy sterilised Toxoplasma gondii tachyzoites maintain metabolic functions and mammalian cell invasion, eliciting cellular immunity and cytokine response similar to natural infection in mice
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DOI：
10.1016/s0264-410x(02)00054-3
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发表时间：
2002-05-15
期刊：
影响因子：
5.5
通讯作者：
de Andrade, HF
中科院分区：
文献类型：
--
作者：
Hiramoto, RM;Galisteo, AJ;de Andrade, HF
200戈伊照射的弓形虫RH速殖子在体外和体内均不能繁殖。在短期培养中，这些寄生虫保持呼吸反应，侵入细胞的能力，并保持蛋白质和核酸的合成。ELISA和蛋白质印迹技术表明，在体液反应的相似性与γ射线照射的速殖子感染的小鼠和动物感染的幼稚寄生虫，并与磺胺嘧啶治疗，高于小鼠免疫与甲脒杀死的速殖子。由弓形虫抗原刺激脾细胞产生的淋巴增殖反应和细胞因子谱（IL-10、IL-12、IFN-γ和TNF-α）与慢性自然感染产生的相似。用辐射速殖子免疫的小鼠在随后的速殖子攻击后存活时间延长，并且在囊肿攻击后显示出最小的脑病理学。辐照速殖子丧失繁殖能力，同时保持代谢功能，这可能为弓形虫病的研究和疫苗开发提供新的工具。(C)2002爱思唯尔科技有限公司版权所有。
200 Gy gamma-irradiated Toxoplasma gondii RH tachyzoites failed to reproduce in vitro and in vivo. In short-term cultures, these parasites maintained a respiratory response, the ability to invade cells and preserved protein and nucleic acid synthesis. ELISA and Western blotting techniques demonstrated the similarity in humoral response between mice infected with gamma-irradiated tachyzoites and animals infected with naive parasites and treated with sulfadoxine, higher than mice immunised with formaldehyde-killed tachyzoites. Splenocyte stimulation by T gondii antigen produced lymphoproliferative response and cytokine profile (IL-10, IL-12, IFN-gamma and TNF- alpha) similar to those produced by chronic natural infection. Mice immunised with irradiated tachyzoites had extended survival times after subsequent tachyzoite challenge, and displayed minimal cerebral pathology after cyst challenge. Irradiated tachyzoites lose their reproductive ability whilst maintaining metabolic function and may provide a novel tool for the study of toxoplasmosis and vaccine development. (C) 2002 Elsevier Science Ltd. All rights reserved.