MOLECULAR AND MICROSCOPIC IDENTIFICATION OF SULFATE-REDUCING BACTERIA IN MULTISPECIES BIOFILMS
MOLECULAR AND MICROSCOPIC IDENTIFICATION OF SULFATE-REDUCING BACTERIA IN MULTISPECIES BIOFILMS
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DOI：
10.1128/aem.58.2.614-623.1992
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发表时间：
1992-02-01
影响因子：
4.4
通讯作者：
STAHL, DA
中科院分区：
文献类型：
--
作者：
AMANN, RI;STROMLEY, J;STAHL, DA
通过选择性聚合酶链反应扩增和荧光显微镜观察，研究了厌氧固定床生物反应器中形成的产硫生物膜的种群结构。通过聚合酶链反应选择性地扩增了居民硫酸盐还原菌共同的16S rRNA区域。扩增产物的序列，参考代表最具特征的硫酸盐还原菌的16S rRNA序列的集合，用于设计通用和特异性杂交探针。这些探针的荧光版本与荧光显微镜结合使用，以可视化特定的硫酸盐还原细菌种群内的发展和建立的生物膜。
The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design both general and specific hybridization probes. Fluorescent versions of these probes were used in combination with fluorescence microscopy to visualize specific sulfate-reducing bacterial populations within developing and established biofilms.