OBSERVATIONS ON A STRAIN OF LEISHMANIA-TROPICA AFTER PROLONGED CULTIVATION - NOTES ON INFECTIVITY AND IMMUNITY
OBSERVATIONS ON A STRAIN OF LEISHMANIA-TROPICA AFTER PROLONGED CULTIVATION - NOTES ON INFECTIVITY AND IMMUNITY
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DOI：
10.1080/00034983.1948.11685360
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发表时间：
1948-01-01
影响因子：
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通讯作者：
ZUCKERMAN, A
中科院分区：
文献类型：
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作者：
ADLER, S;ZUCKERMAN, A
该菌株的历史如下。1925年10月26日在杰里科捕获了一只雌性帕帕塔斯白蛉，11月2日在耶路撒冷进行了解剖。在这两个日期之间，昆虫没有进食的机会。解剖时发现细胞菌的严重感染从长鼻延伸到直肠。贲门的上部完全被鞭毛虫堵塞，大量鞭毛虫附着在食管瓣和上皮上。食管憩室在其与食管交界处后面的一团几乎是固体的鞭毛虫上收缩，将这种白蛉的材料接种到志愿者左前臂的两个划痕点上。11月27日，患者注意到接种点部位出现两个丘疹，由于瘙痒，他的注意力被吸引到这些丘疹上。他在11月29日接受了检查，在丘疹的涂片中发现了许多利什曼-多诺万体。随后病变发展为典型的东方疮。从这种情况下获得的菌株，并标记为“Ber”，此后一直在洛克血清和洛克血琼脂上培养。在过去的22年里，它在这些媒体上经历了大约800次。分离后2年，通过在已知浓度的鞭毛虫悬液上用膜喂食昆虫，检查了该菌株在沙地中的行为，发现它是L. papatasii的典型地方菌株。热带，即第三天后，鞭毛虫已上升到食道瓣附近。还发现（Adler和Theodor，1926年），通过血清学方法，该菌株与其他巴勒斯坦菌株无法区分。
The history of the strain is as follows. A female Phlebotomus papatasii was caught in Jericho on October 26th, 1925, and was dissected in Jerusalem on November 2nd. Between these two dates the insect had no opportunity of feeding. On dissection a heavy infection of leptomonas was found extending from the proboscis to the rectum. The upper part of the cardia was completely choked by flagellates, large numbers being attached to the oesophageal valve and epithelium. The oesophageal diverticulum was found contracting on an almost solid mass of flagellates immediately behind its junction with the oesophagus.Material from this sandfly was inoculated into two scarified points on the left forearm of a volunteer. On November 27th the patient noticed two papules on the site of the inoculated points, his attention being drawn to them on account of itching. He was examined on November 29th and numerous Leishman-Donovan bodies were found in smears from the papules. Subsequently the lesions developed into typical oriental sores. t The strain obtained from this case, and labelled'Ber,'has since been maintained continuously in culture on Locke-serum and Locke-blood-agar. During the last 22 years it has undergone about 800 passages on these media. Two years after its isolation the behaviour of the strain in sandfties, P. papatasii, was examined by feeding the insects through a membrane on suspensions of flagellates of known concentration, and it was found to be typical of local strains of L. tropica, ie, after the third day the flagellates had ascended to the neighbourhood of the oesophageal valve. It was also found (Adler and Theodor, 1926) that the strain was indistinguishable from other Palestinian strains by serological methods.