A conserved Bcd1 interaction essential for box C/D snoRNP biogenesis
A conserved Bcd1 interaction essential for box C/D snoRNP biogenesis
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DOI：
10.1074/jbc.ra119.010222
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发表时间：
2019-11-29
影响因子：
4.8
通讯作者：
Ghalei, Homa
中科院分区：
文献类型：
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作者：
Khoshnevis, Sohail;Dreggors, R. Elizabeth;Ghalei, Homa
核糖体的产生和蛋白质的准确翻译需要rRNA的精确修饰和加工。小核仁核糖核蛋白（snoRNP）指导rRNA的折叠、修饰和加工，因此对所有真核细胞都至关重要。Bcd 1是真核生物中功能保守的一种重要锌指HIT蛋白，它被认为是box C/D snoRNP生物合成的早期调节因子，并通过一种未知的机制控制box C/D snoRNA的稳态水平。使用遗传学和生物化学方法的组合，在这里，我们发现了一个保守的N-末端基序Bcd 1从酿酒酵母，这是需要与盒C/D snoRNA和核心snoRNP蛋白，Snu 13的相互作用。我们表明，这两个Bcd 1？snoRNA和Bcd 1？Snu 13相互作用对snoRNP组装和核糖体生物合成至关重要。我们的研究结果提供了对Bcd 1相互作用的机制性见解，Bcd 1相互作用可能控制snoRNP成熟的早期步骤，并有助于这种蛋白质在维持细胞中snoRNA的稳态水平方面发挥重要作用。
Precise modification and processing of rRNAs are required for the production of ribosomes and accurate translation of proteins. Small nucleolar ribonucleoproteins (snoRNPs) guide the folding, modification, and processing of rRNAs and are thus critical for all eukaryotic cells. Bcd1, an essential zinc finger HIT protein functionally conserved in eukaryotes, has been implicated as an early regulator for biogenesis of box C/D snoRNPs and controls steady-state levels of box C/D snoRNAs through an unknown mechanism. Using a combination of genetic and biochemical approaches, here we found a conserved N-terminal motif in Bcd1 from Saccharomyces cerevisiae that is required for interactions with box C/D snoRNAs and the core snoRNP protein, Snu13. We show that both the Bcd1?snoRNA and Bcd1?Snu13 interactions are critical for snoRNP assembly and ribosome biogenesis. Our results provide mechanistic insight into Bcd1 interactions that likely control the early steps of snoRNP maturation and contribute to the essential role of this protein in maintaining the steady-state levels of snoRNAs in the cell.