UV-independent induction of beta defensin 3 in neonatal human skin explants.
UV-independent induction of beta defensin 3 in neonatal human skin explants.
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DOI：
10.12688/f1000research.5794.2
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
D'Orazio J
中科院分区：
文献类型：
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作者：
Wolf Horrell E;D'Orazio J
为了确定紫外线辐射对人皮肤中β-防御素3 （BD3）表达的影响，从计划包皮环切术的新生男婴中获得新鲜分离的UV-naïve皮肤。将体外真皮侧朝下放置于RPMI培养基上的皮肤外植体暴露于0.5 kJ/ m2 UVB中，并在辐射后72小时对外植体进行活检。qRTPCR检测mRNA表达，归一化为tata结合蛋白。将每个时间点的BD3表达与每个皮肤样本中0时间的未处理对照进行比较。BD3诱导的时间和程度在个体之间存在广泛的差异，并且不能通过皮肤色素表型预测，这表明BD3的诱导不受表皮黑色素化的影响。然而，模拟辐照时间过程显示，在多个供体中，与紫外线无关的BD3 mRNA增加，而紫外线辐射治疗没有进一步增强，这表明除紫外线损伤外的其他因素促进了皮肤外植体中BD3表达的增加。我们得出的结论是，在标准培养条件下处理和维持的人皮肤外植体中，BD3的表达是不依赖于紫外线的，并且新生儿皮肤外植体不是研究紫外线对整个人皮肤诱导BD3的影响的合适模型。
In order to determine the effect of UV radiation on β-defensin 3 (BD3) expression in human skin, freshly-isolated UV-naïve skin was obtained from newborn male infants undergoing planned circumcision. Skin explants sustained ex vivo dermis side down on RPMI media were exposed to 0.5 kJ/m 2 UVB, and biopsies were taken from the explant through 72 hours after radiation. mRNA expression was measured by qRTPCR and normalized to TATA-binding protein. BD3 expression at each time point was compared with an untreated control taken at time 0 within each skin sample. Extensive variability in both the timing and magnitude of BD3 induction across individuals was noted and was not predicted by skin pigment phenotype, suggesting that BD3 induction was not influenced by epidermal melanization. However, a mock-irradiated time course demonstrated UV-independent BD3 mRNA increases across multiple donors which was not further augmented by treatment with UV radiation, suggesting that factors other than UV damage promoted increased BD3 expression in the skin explants. We conclude that BD3 expression is induced in a UV-independent manner in human skin explants processed and maintained in standard culture conditions, and that neonatal skin explants are an inappropriate model with which to study the effects of UV on BD3 induction in whole human skin.