One-Pot Synthesis of Aptamer-Functionalized Silver Nanoclusters for Cell-Type-Specific Imaging
One-Pot Synthesis of Aptamer-Functionalized Silver Nanoclusters for Cell-Type-Specific Imaging
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用于细胞类型特异性成像的适体功能化银纳米团簇的一锅法合成
DOI：
10.1021/ac3003402
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发表时间：
2012-05-01
影响因子：
7.4
通讯作者：
Zhu, Jun-Jie
中科院分区：
文献类型：
--
作者：
Li, Jingling;Zhong, Xiaoqin;Zhu, Jun-Jie
作为一类新兴的荧光金属纳米团簇，以荧光素为模板的银纳米团簇（Ag NCs）在生物领域有着广泛的应用前景。然而，弱的荧光发射和差的细胞膜渗透性限制了银纳米颗粒的进一步细胞内应用。AS 1411是一种抗增殖的富含G的磷酸二酯寡核苷酸，目前是一种正在进行II期临床试验的抗癌药物。在本文中，我们提出了一种策略，通过一锅法合成具有优异荧光的AS 1411功能化的银纳米粒子。共聚焦激光扫描显微镜和Z轴扫描证实，AS 1411功能化的Ag NCs可以内化到MCF-7人乳腺癌细胞中，并且能够特异性地将细胞核染成红色。结果表明，银纳米粒具有良好的细胞相容性，对MCF-7人乳腺癌细胞的生长抑制作用优于AS 1411。此外，用于合成Ag NCs的寡核苷酸支架的通用设计扩展到其他适体，例如Sgc 8 c和粘蛋白1适体。由于该荧光平台的合成过程简单，且具有特异性靶点识别能力，因此有望拓宽银纳米粒子在生物传感和生物成像方面的应用。
As an emerging category of fluorescent metal nanoclusters, oligonucleotide-templated silver nanoclusters (Ag NCs) have attracted a lot of interest and have shown wide application in biorelated disciplines. However, the weak fluorescence emission and poor permeability to cell membranes tethered further intracellular applications of Ag NCs. AS 1411 is an antiproliferative G-rich phosphodiester oligonucleotide and currently an anticancer agent under phase II clinical trials. Herein, we present a strategy to synthesize AS1411-functionalized Ag NCs with excellent fluorescence through a facile one-pot process. Confocal laser scanning microscopy and Z-axis scanning confirmed that the AS1411-functionalized Ag NCs could be internalized into MCF-7 human breast cancer cells and were able to specifically stain nuclei with red color. To our surprise, 3-[4,5-dimethylthiazol-z-yl]-2,5-diphenyltetrazolium bromide (MTT) assay demonstrated the Ag NCs were cytocompatible and showed better inhibition effects than pure AS 1411 on MCF-7 human breast cancer cells. In addition, a universal design of the oligonucleotide scaffold for synthesis of Ag NCs was extended to other aptamers, such as Sgc8c and mucin 1 aptamer. Due to the facile synthesis procedure and capability of specific target recognition, this fluorescent platform will potentially broaden the applications of Ag NCs in biosensing and biological imaging.