Characteristics of Escherichia coli isolated from broiler chickens with colibacillosis in commercial farms from a common hatchery
Characteristics of Escherichia coli isolated from broiler chickens with colibacillosis in commercial farms from a common hatchery
复制标题
DOI：
10.3382/ps/pex167
复制
发表时间：
2017-10-01
期刊：
影响因子：
4.4
通讯作者：
Murase, T.
中科院分区：
文献类型：
--
作者：
Ozaki, H.;Matsuoka, Y.;Murase, T.
为了解肉鸡大肠杆菌病的流行病学特征，从4个商品化鸡场的肉鸡心包炎和肝炎周围病变中分离到83株大肠杆菌，5株分离自25只消瘦鸡的卵黄囊内容物中，和从向上述4个商业农场供应雏鸡的孵化场获得的4个粪便分离物进行遗传学和细菌学表征。采用脉冲场凝胶电泳（PFGE），共将92个菌株分为33个脉冲型。在不同采样时间从多个农场的孵化场样品和受影响的肉鸡中获得的分离株中观察到相同的脉型。17个代表性的菌株，没有共同的起源属于6脉型和额外的27个菌株与其他脉型用于进一步的实验。具有相同pulsotypes的分离株表现出共同的性状毒力相关基因，脂多糖核心类型，和系统发育组。其中9株血清学分型为O125，并伴有各种类型的H抗原，3株血清学分型为O25：H4。在对第三代头孢菌素头孢噻呋（CTF）耐药的27株分离株中，bla（CTX-M-2）、bla（CMY-2）、bla（CTX-M-14）和bla（CTX-M-65）基因分别在15、8、3和1株分离株中发现，另一株对CTF耐药的分离株同时具有bla（CTX-M-2）和bla（CMY-2）基因。在16株携带bla（CTX-M-2）基因的分离株中，有12株进行了染色体定位。质粒介导的喹诺酮类耐药基因oqxAB和aac（6 '）-Ib-cr分别在2株和3株中检出。结合实验显示，bla（CTX-M-2）（4个分离株）、bla（CTX-M-14）（3个分离株）、bla（SHV-12）（1个分离株）和oqxAB（2个分离株）基因被转移。我们的数据表明E. pusotype相同的大肠杆菌菌株引起了大肠杆菌病的发生，接合质粒上的耐药基因和整合到染色体上的耐药基因可能在禽致病性大肠杆菌中传播。大肠杆菌菌株。
To investigate the epidemiologic aspects of colibacillosis in broiler chickens, 83 Escherichia coli isolates obtained from the pericarditis and perihepatitis lesions in broiler chickens from 4 commercial farms, 5 isolates recovered from 5 samples of yolk sac contents that were pooled from 25 emaciated chicks, and 4 fecal isolates obtained from a hatchery that supplied chicks to the 4 commercial farms mentioned above were genetically and bacteriologically characterized. Using pulsed-field gel electrophoresis (PFGE), a total of 92 isolates were classified into 33 pulsotypes. Identical pulsotypes were observed in isolates obtained from hatchery samples and the affected broiler chickens on multiple farms at various sampling times. Seventeen representative isolates with no common origin belonging to 6 pulsotypes and an additional 27 isolates with the other pulsotypes were used for further experiments. Isolates with identical pulsotypes exhibited common traits for virulence-associated genes, lipopolysaccharide core types, and phylogenetic groups. Nine of the isolates were serologically typed as O125 with various types of H antigens and 3 were typed as O25:H4. In the 27 isolates resistant to ceftiofur (CTF), which is a third generation cephalosporin, the bla(CTX-M-2), bla(CMY-2), bla(CTX-M-14), bla(CTX-M-65) genes were found in 15, 8, 3, and 1 isolate(s), respectively, and another isolate resistant to CTF had both the bla(CTX-M-2) and the bla(CMY-2) genes. In the 16 isolates with the bla(CTX-M-2) gene, the chromosomal location of the gene was identified in 12 isolates. The plasmid-mediated quinolone resistance genes, oqxAB and aac(6')-Ib-cr, were found in 2 and 3 isolates, respectively. Conjugation experiments revealed that the bla(CTX-M-2) (4 isolates), bla(CTX-M-14) (3 isolates), bla(SHV-12) (1 isolate), and oqxAB (2 isolates) genes were transferred. Our data suggest that E. coli strains with identical pulsotypes had been caused the incidences of colibacillosis and that the antimicrobial resistance genes on conjugative plasmids and those integrated into the chromosome may be spread among avian pathogenic E. coli strains in multiple farms.