QTL analysis for low temperature tolerance of wild potato species Solanum commersonii in natural field trials
QTL analysis for low temperature tolerance of wild potato species Solanum commersonii in natural field trials
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野生马铃薯自然田间耐低温性QTL分析
DOI：
10.1016/j.scienta.2022.111689
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发表时间：
2022-11-25
影响因子：
4.3
通讯作者：
Cai，Xingkui
中科院分区：
文献类型：
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作者：
Dong，Jianke;Li，Jingwen;Cai，Xingkui
马铃薯品种（Solanum tuberosumL.）对寒冷和霜冻很敏感。低温对马铃薯生长发育和产量有不利影响。然而，目前对抗冻基因座的遗传检测还很缺乏。在这里，我们报告了一个由二倍体耐寒野生物种杂交构建的BC1群体。与二倍体对冷敏感的野生种有关。verrucosum。通过4年的自然田间霜冻试验，确定了后代的抗冻性。结合大量分离分析（BSA）技术和传统的数量性状位点（QTL）定位，在Chr02和Chr11上检测到4个高度相关的QTL。此外，我们构建了Chr02的部分遗传连锁图谱，并在Chr02上检测到两个QTL1和QTL2。据报道，位于候选区间的四个基因与对非生物胁迫的耐受性有关，并且在抗性和敏感性之间表达显着差异。综上所述，我们的研究结果还表明，植物在不同低温条件下的抗冻性是由不同的基因座控制的。
Potato cultivars (Solanum tuberosumL.) are delicate to cold and frost. Low temperatures have a negative impact on potato growth, development and yield. However, genetic detection of frost tolerance loci is currently lacking. Here, we report a BC1 population that was constructed by crossing the diploid cold-resistant wild speciesS. commersoniiwith the diploid cold-sensitive wild speciesS. verrucosum. The frost tolerance of the resulting offspring was determined through four years of natural field frost experiments. Together with bulked segregant analysis (BSA) technology and traditional quantitative trait locus (QTL) mapping, four highly related QTLs were detected on Chr02 and Chr11. Furthermore, we constructed a partial genetic linkage map of Chr02, and two QTLs (QTL1 and QTL2) were detected on Chr02. Four genes located in candidate intervals have been reported to be associated with tolerance to abiotic stress and were significantly differentially expressed among the resistant and sensitive. Taken together, our results also suggest that the frost resistance of plants under variable low-temperature conditions is controlled by different loci.