Oral Administration of Ethanolamine Glycerophospholipid Containing a High Level of Plasmalogen Improves Memory Impairment in Amyloid β-Infused Rats
Oral Administration of Ethanolamine Glycerophospholipid Containing a High Level of Plasmalogen Improves Memory Impairment in Amyloid β-Infused Rats
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DOI：
10.1007/s11745-017-4260-3
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发表时间：
2017-07
期刊：
影响因子：
1.9
通讯作者：
Shinji Yamashita;M. Hashimoto;A. Haque;K. Nakagawa;M. Kinoshita;O. Shido;T. Miyazawa
中科院分区：
文献类型：
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作者：
Shinji Yamashita;M. Hashimoto;A. Haque;K. Nakagawa;M. Kinoshita;O. Shido;T. Miyazawa
乙醇胺plasmalogen （PlsEtn）是神经元膜中的一种主要磷脂[60-90摩尔%的乙醇胺甘油磷脂（EtnGpl）]，在阿尔茨海默病（AD）患者的大脑中特异性减少。本研究调查了PlsEtn如何影响AD动物模型中的认知缺陷和脂质组成。将淀粉样蛋白β (Aβ)注入AD模型大鼠脑室，分为3组。对照组、蛋类组和海鞘组分别口服对照物、蛋黄EtnGpl （260 μmol /kg BW/day, 10 μmol /kg BW/day）或海鞘脏器EtnGpl （260 μmol /kg BW/day, 209 μmol /kg BW/day）。给药4周后，注射a β的大鼠在8臂径向迷宫中进行学习能力测试。海鞘脏器EtnGpl的使用提高了参考和工作记忆相关的学习能力。在脂质分析中，与其他组相比，海鞘组在血浆、红细胞和肝脏中显示出更高的PlsEtn水平。此外，虽然在包括胆碱在内的总醛脂原水平上没有差异，但海鞘组的大脑皮层18:00 ol/22:6-PlsEtn水平显著高于海鞘组。大脑皮层18:00 ol/22:6-PlsEtn水平与工作记忆相关的学习能力相关。此外，大脑皮层18:00 ol/22:6-PlsEtn水平与红细胞和肝脏中的plsetn水平呈正相关。总之，膳食PlsEtn，特别是含有22:6n-3（二十二碳六烯酸，DHA）的膳食PlsEtn可能通过改变大脑中的脂质组成来改善AD的学习缺陷。
Ethanolamine plasmalogen (PlsEtn), a major phospholipid in neuronal membranes [60–90 mol% of ethanolamine glycerophospholipid (EtnGpl)], is specifically decreased in brains from patients with Alzheimer’s disease (AD). The present study investigated how PlsEtn administration affects cognitive deficits and lipid composition in an animal model of AD. AD model rats were infused with amyloid-β (Aβ) into the cerebral ventricle and divided into three groups. Control, Egg, and Ascidian groups were then orally administered vehicle, egg yolk EtnGpl (260 μmol as EtnGpl/kg BW/day; 10 μmol as PlsEtn/kg BW/day), or ascidian viscera EtnGpl (260 μmol as EtnGpl/kg BW/day; 209 μmol as PlsEtn/kg BW/day), respectively. After 4 weeks of dosing, Aβ-infused rats were tested for learning ability in an 8-arm radial maze. The administration of ascidian viscera EtnGpl improved both reference and working memory-related learning abilities. In lipid analysis, the Ascidian group showed higher levels of PlsEtn species in the plasma, erythrocytes, and liver when compared to other groups. In addition, although there were no differences at levels of total plasmalogen including choline plasmalogen, the Ascidian group had significantly higher levels of 18:0ol/22:6-PlsEtn in the cerebral cortex. These levels of 18:0ol/22:6-PlsEtn in the cerebral cortex were correlated with working memory-related learning ability. Moreover, 18:0ol/22:6-PlsEtn levels in the cerebral cortex showed positive correlations with those in the erythrocytes and liver. In summary, dietary PlsEtn, especially that with 22:6n-3 (docosahexaenoic acid, DHA), may ameliorate learning deficiencies in AD by altering lipid composition in the brain.