DNA polymorphisms indicate loss of heterozygosity for chromosome 11 of D98AH2 cells.
DNA polymorphisms indicate loss of heterozygosity for chromosome 11 of D98AH2 cells.
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DNA 多态性表明 D98AH2 细胞 11 号染色体杂合性丢失。
DOI：
10.1159/000132236
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Klinger,HP
中科院分区：
文献类型：
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作者：
Kaelbling,M;Roginski,RS;Klinger,HP
对正常二倍体细胞与致瘤性D98 AH 2（D98）细胞融合的细胞杂交体的研究表明，正常细胞的人类11号染色体携带致瘤性抑制信息。宫颈癌衍生的D98（HeLa）细胞含有两个拷贝的11号染色体。在这项研究中，用9种限制性内切酶中的一种消化D98细胞的DNA，并与11号染色体短臂上高度多态性区域的5种DNA探针杂交，对DNA的限制性片段长度多态性进行分析，在胰岛素（INS）、哈维小鼠肉瘤病毒1（HRAS 1）和β-珠蛋白簇（HBBC）区域未检测到杂合性。个体在所有这些基因座上纯合的可能性很低，这表明Nos. D98细胞中有11个是仅一个原始同源物的拷贝，或至少是所检查的短臂节段的拷贝。这表明D98细胞可以表达与致瘤性相关的改变或丢失的基因，即使这种改变是隐性的。在致瘤性抑制的杂交体中，11的正常细胞可以补充D98细胞的这种遗传缺陷。
Studies with cell hybrids of normal diploid cells fused with tumorigenic D98AH2 (D98) cells had implicated human chromosome 11 of a normal cell as carrying tumorigenicity suppressing information. The cervical carcinoma-derived D98 (HeLa) cells contain two copies of chromosome 11. In this study, analysis of restriction fragment length polymorphism of DNA from D98 cells digested with one of nine restriction endonucleases and hybridized with five DNA probes for highly polymorphic regions on the short arm of chromosome 11 detected no heterozygosity at the insulin (INS), Harvey murine sarcoma virus 1 (HRAS1), and the β-globin cluster (HBBC) regions. The low probability of an individual being homozygous at all these loci suggests that the Nos. 11 of the D98 cells are both copies of only one of the original homologs, or at least of the short arm segment examined. This indicates that the D98 cells could express altered or lost genes associated with tumorigenicity, even if such changes were recessive. In tumorigenically suppressed hybrids the Nos. 11 of the normal cell could then be complementing this genetic defect of the D98 cells.