Modulation of enzyme activities in isolated lymphocyte plasma membranes by enzymatic modification of phospholipid fatty acids.
Modulation of enzyme activities in isolated lymphocyte plasma membranes by enzymatic modification of phospholipid fatty acids.
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通过磷脂脂肪酸的酶促修饰来调节分离的淋巴细胞质膜中的酶活性。
DOI：
10.1016/s0021-9258(19)68449-8
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
K. Resch
中科院分区：
文献类型：
--
作者：
M. Szamel;K. Resch
外源性活化ligandsSubstrates--l-palmitoyl-sn-3-glycerophosphocholine，棕榈酰辅酶A的结合调节淋巴细胞的分化或生长增殖，油酰辅酶A是从Sigma、L-[L-“C]棕榈酰-sn-甘油-3-磷胆碱和[L-‘4C]油酰辅酶A中获得的。根据Reitz等人(8)以亚油酰氯或花生四烯酰氯(NuChek Prep)和辅酶A(Boehringer)为底物合成了亚油酰基辅酶A和花生四烯基辅酶A。反应结束后，立即加入1pmo1/2pmo1/2pmoL的酰基辅酶A(2，6-二叔丁基-4-甲基苯酚)作为抗氧化剂。
MATERIALS AND METHODSDifferentiation or growth and proliferation of lymphocytes are regulated by the binding of exogenous activating ligandsSubstrates--l-palmitoyl-sn-3-glycerophosphocholine, palmitoyl coenxyme A, and oleoyl coenzyme A were obtained from Sigma, l-[l-“C] palmitoyl-sn-glycero-3-phosphocholine and [l-‘4C] oleoyl coenzyme A from Amersham Buchler. Linoleoyl coenzyme A and arachidonoyl coenxyme A were synthesized according to Reitz et al.(8) using linoleoyl chloride or arachidonoyl chloride (NuChek Prep) and coenxyme A (Boehringer) as substrates. Immediately after termination of the reaction, 1 pmo1/2 pmol of acyl-coA(2, 6-ditert-butyl-4-methylphenol) were added as an antioxidant.