Smaller 145th residue makes fluorescent protein non-fluorescent: Fluorescence lifetimes of enhanced yellow fluorescent protein (eYFP) Y145 mutants and H148 mutants
Smaller 145th residue makes fluorescent protein non-fluorescent: Fluorescence lifetimes of enhanced yellow fluorescent protein (eYFP) Y145 mutants and H148 mutants
复制标题
DOI：
10.1016/j.cplett.2014.11.014
复制
发表时间：
2015-01-02
影响因子：
2.8
通讯作者：
Takeda, Yuri
中科院分区：
文献类型：
--
作者：
Hosoi, Haruko;Hazama, Sakiko;Takeda, Yuri
为了回答为什么荧光蛋白是荧光的这个基本问题，我们对增强型黄色荧光蛋白（eYFP）、其19种不同的Y145突变体和其19种不同的H148突变体进行了荧光寿命测量。Y145突变体的寿命在3.4 ns至82 ps的宽范围内变化很大，而H148突变体的寿命变化相对较小。该结果表明，单个第145侧链可以控制eYFP的荧光效率。此外，发现第145位侧链的空间效应是控制荧光效率的原因。(C)2014爱思唯尔有限公司版权所有。
To answer the fundamental question of why fluorescent proteins are fluorescent, we performed fluorescence lifetime measurements of enhanced yellow fluorescent protein (eYFP), its 19 different Y145 mutants, and its 19 different H148 mutants. The lifetimes of the Y145 mutants varied drastically over a wide range of 3.4 ns to 82 ps, whereas the variation in the lifetime of the H148 mutants was relatively small. This result demonstrates that the single 145th side chain can control the fluorescence efficiency of eYFP. Furthermore, it was found that the steric effect of the 145th side chain is responsible for controlling the fluorescence efficiency. (C) 2014 Elsevier B.V. All rights reserved.