Deep in vivo photoacoustic imaging of mammalian tissues using a tyrosinase-based genetic reporter
Deep in vivo photoacoustic imaging of mammalian tissues using a tyrosinase-based genetic reporter
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DOI：
10.1038/nphoton.2015.22
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发表时间：
2015-04-01
期刊：
影响因子：
35
通讯作者：
Beard, Paul
中科院分区：
文献类型：
--
作者：
Jathoul, Amit P.;Laufer, Jan;Beard, Paul
光声成像允许在超过光学显微镜的深度处的基于吸收的高分辨率光谱体内成像。直到最近，光声成像在很大程度上被限制为通过内源性血红蛋白造影剂来可视化脉管系统，除非施用外源性造影剂，否则大多数非血管化组织保持不可见。遗传编码的光声对比度是有吸引力的，因为它允许选择性标记细胞，允许研究，例如，特定的遗传表达，细胞生长或更复杂的生物学行为在体内。在这项研究中，我们报告了一种新的光声成像扫描仪和酪氨酸酶为基础的报告系统，使人类细胞系合成吸收色素真黑素，从而提供强大的光声对比。在接近10 mm的深度以低于100 μ m的空间分辨率在体内获得由植入小鼠中的酪氨酸酶表达细胞形成的异种移植物的详细三维图像。该方案是研究深层哺乳动物组织中细胞和遗传过程的有力工具。
Photoacoustic imaging allows absorption-based high-resolution spectroscopic in vivo imaging at a depth beyond that of optical microscopy. Until recently, photoacoustic imaging has largely been restricted to visualizing the vasculature through endogenous haemoglobin contrast, with most non-vascularized tissues remaining invisible unless exogenous contrast agents are administered. Genetically encodable photoacoustic contrast is attractive as it allows selective labelling of cells, permitting studies of, for example, specific genetic expression, cell growth or more complex biological behaviours in vivo. In this study we report a novel photoacoustic imaging scanner and a tyrosinase-based reporter system that causes human cell lines to synthesize the absorbing pigment eumelanin, thus providing strong photoacoustic contrast. Detailed three-dimensional images of xenografts formed of tyrosinase-expressing cells implanted in mice are obtained in vivo to depths approaching 10 mm with a spatial resolution below 100 mu m. This scheme is a powerful tool for studying cellular and genetic processes in deep mammalian tissues.