Bovine leukemia virus p24 antibodies reflect blood proviral load
Bovine leukemia virus p24 antibodies reflect blood proviral load
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DOI：
10.1186/1746-6148-8-187
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发表时间：
2012-10-09
影响因子：
2.6
通讯作者：
Trono, Karina
中科院分区：
文献类型：
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作者：
Gutierrez, Geronimo;Carignano, Hugo;Trono, Karina
背景：牛白血病病毒（BLV）是世界性分布，在阿根廷高度流行。在预防BLV传播的策略中，基于根据其前病毒负荷（PVL）选择性隔离动物的控制计划对我们的乳制品生产系统是有希望的。这项工作的目的是研究血液PVL和抗体水平之间的关系，以确定个体体液应答（即抗p24或抗全BLV颗粒）是否可用作血液感染水平的标志物，从而有助于招募在自然条件下可能造成较低传播风险的动物。研究的15个农场的p24抗体的流行率超过66%。在取自牛群T1和51的所有样本（n = 196）中分析了p24和全BLV抗体的流行率以及PVL定量。ROC分析显示，p24抗体的AUC高于全BLV抗体（Z（反应性）：3.55，P < 0.001; Z（滴度）：2.88，P < 0.01），因此，在牛群51中预测前病毒载量状态的性能更好。在牛群T1中，未发现p24和全BLV抗体的性能存在显著差异。两个猪场PVL值与p24抗体反应性呈显著正相关（r（T1）= 0.7，P < 0.001; r（51）= 0.71，P < 0.0001）。将分析扩展到其他13个农场的全部弱p24抗体反应者（n = 311）。弱p24反应器中高PVL反应器的平均值为17.38%（SD = 8.92）。5/15个猪场中p24弱反应者的高PVL率低于10%。结论：体液免疫反应反映了体内感染水平，具有一定的流行病学应用价值。虽然使用实时PCR定量评估血液前病毒载量昂贵且技术要求高，但通过ELISA测量血液中的抗体相对简单，因此可以构成BLV控制干预策略中具有成本效益的工具，特别是在高度感染的牛群中，例如阿根廷奶牛。
Background: Bovine leukemia virus (BLV) is worldwide distributed and highly endemic in Argentina. Among the strategies to prevent BLV dissemination, a control plan based on the selective segregation of animals according to their proviral load (PVL) is promising for our dairy productive system. The objective of this work was to study the relationship between the blood PVL and the antibody level, in order to identify whether the individual humoral response, i.e. the anti-p24 or anti-whole-BLV particle, could be used as a marker of the blood level of infection and thus help to recruit animals that may pose a lower risk of dissemination under natural conditions.Results: The prevalence of p24 antibodies on the 15 farms studied was over 66%. The prevalence of p24 and whole-BLV antibodies and PVL quantification were analyzed in all the samples (n = 196) taken from herds T1 and 51. ROC analysis showed a higher AUC for p24 antibodies than whole-BLV antibodies (Z(reactivity): 3.55, P < 0.001; Z(titer): 2.88, P < 0.01), and as consequence a better performance to predict the proviral load status in herd 51. No significant differences were found between the performance of p24 and whole-BLV antibodies in herd T1. A significant positive correlation was observed between PVL values and p24 antibody reactivity in both farms (r (T1) = 0.7, P < 0.001, r (51) = 0.71, P < 0.0001). The analysis was extended to the whole number of weak p24 antibody reactors (n = 311) of the other 13 farms. The mean of high PVL reactors within weak p24 reactors was 17.38% (SD = 8.92). In 5/15 farms, the number of weak p24 reactors with high PVL was lower than 10%.Conclusions: We found that the humoral response reflected the level of in vivo infection, and may therefore have useful epidemiological applications. Whereas the quantitative evaluation of blood proviral load using real-time PCR is expensive and technically demanding, the measurement of antibodies in blood by ELISA is relatively straightforward and could therefore constitute a cost-effective tool in a BLV control intervention strategy, especially in highly infected herds such as Argentinean dairy ones.