Identification of androgen, estrogen and progesterone receptor mRNAs in the eye
Identification of androgen, estrogen and progesterone receptor mRNAs in the eye
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DOI：
10.1034/j.1600-0420.2000.078002146.x
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发表时间：
2000-04-01
期刊：
影响因子：
--
通讯作者：
Sullivan, DA
中科院分区：
文献类型：
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作者：
Wickham, LA;Gao, JP;Sullivan, DA
目的：先前的研究已经证明性类固醇对许多眼组织的结构和功能产生显著影响，为了开始探索这种激素作用的潜在基础，我们检测了眼的各种前部和后部组织是否含有雄激素、雌激素和孕激素受体mRNA。从成年雄性和雌性大鼠、兔和人获得组织样品，处理用于分离总RNA，并通过RT-PCR、琼脂糖凝胶电泳和Southern印迹杂交进行分析，结果：雄激素、雌激素和/或孕激素受体mRNA在大鼠、兔和人的泪腺、泪腺腺泡上皮细胞、睑板腺、眼睑、睑球结膜、角膜、虹膜/睫状体、透镜、视网膜/葡萄膜、视网膜/脉络膜和视网膜色素上皮细胞中均有表达。我们的研究结果表明，性类固醇受体mRNA存在于各种眼组织，并建议这些网站可能代表雄激素，雌激素和/或孕激素的靶器官。
Purpose: Previous research has demonstrated that sex steroids exert a significant influence on the structure and function of numerous ocular tissues, To begin to explore the underlying basis of this hormone action, we examined whether various anterior and posterior tissues of the eye contain androgen, estrogen and progesterone receptor mRNAs,Methods: Tissue samples were obtained from adult male and female rats, rabbits and humans, processed for the isolation of total RNA and analyzed by RT-PCR, agarose gel electrophoresis and Southern blot hybridization, All PCR amplifications included positive and negative controls.Results: Our findings showed that androgen, estrogen and/or progesterone receptor mRNAs are present in the lacrimal gland, lacrimal gland acinar epithelial cells, meibomian gland, lid, palpebral and bulbar conjunctivae, cornea, iris/ciliary body, lens, retina/uvea, retina/choroid and retinal pigment epithelial cells of rats rabbits or humans.Conclusions: Our findings demonstrate that sex steroid receptor mRNAs exist in a variety of ocular tissues and suggest that these sites may represent target organs for androgens estrogens and/or progestins.