The CLAMP protein links the MSL complex to the X chromosome during Drosophila dosage compensation
The CLAMP protein links the MSL complex to the X chromosome during Drosophila dosage compensation
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DOI：
10.1101/gad.214585.113
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发表时间：
2013-07-15
影响因子：
10.5
通讯作者：
Larschan, Erica
中科院分区：
文献类型：
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作者：
Soruco, Marcela M. L.;Chery, Jessica;Larschan, Erica
果蝇雄性特异性致死（MSL）剂量补偿复合物增加单个雄性X染色体上的转录水平，使其等于XX雌性中的转录水平。然而，尚不清楚MSL复合物如何与其DNA识别元件连接，这是剂量补偿的关键第一步。在这里，我们表明，以前未知的锌指蛋白，CLAMP（染色质连接的适配器MSL蛋白），作为MSL复合物和X染色体之间的第一个链接的功能。CLAMP直接与MSL复合物DNA识别元件结合，并且是MSL复合物募集所需的。CLAMP的发现确定了剂量补偿的染色体特异性靶向所需的关键因素，为后生动物基因组内如何形成协调基因调控的亚核结构域提供了新的见解。
The Drosophila male-specific lethal (MSL) dosage compensation complex increases transcript levels on the single male X chromosome to equal the transcript levels in XX females. However, it is not known how the MSL complex is linked to its DNA recognition elements, the critical first step in dosage compensation. Here, we demonstrate that a previously uncharacterized zinc finger protein, CLAMP (chromatin-linked adaptor for MSL proteins), functions as the first link between the MSL complex and the X chromosome. CLAMP directly binds to the MSL complex DNA recognition elements and is required for the recruitment of the MSL complex. The discovery of CLAMP identifies a key factor required for the chromosome-specific targeting of dosage compensation, providing new insights into how subnuclear domains of coordinate gene regulation are formed within metazoan genomes.