Neonatal vitamin A administration increases intramuscular fat by promoting angiogenesis and preadipocyte formation
Neonatal vitamin A administration increases intramuscular fat by promoting angiogenesis and preadipocyte formation
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新生儿维生素 A 给药通过促进血管生成和前脂肪细胞形成来增加肌内脂肪
DOI：
10.1016/j.meatsci.2022.108847
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发表时间：
2022
期刊：
影响因子：
7.1
通讯作者：
Bo Wang
中科院分区：
文献类型：
--
作者：
Xiaoxiao Yu;Yannan Ma;Yanhui Luo;Gaojian Tang;Zongyou Jiang;Jiashan Zhang;Boping Ye;Zhongzuo Huang;Yicheng Luo;Min Du;Bo Wang
为探讨维生素A对肉牛大理石花纹脂肪发育的影响及其机制，在出生和1月龄时分别对安格斯阉牛注射维生素A，并在体外研究维甲酸（RA）对肌内基质血管（SVF）细胞血管生成和成脂的影响。结果显示，维生素A给药增加了肌内PDGFRα+脂肪祖细胞，提高了肌内SVF细胞的成脂潜力，并显著上调了VEGFA。在屠宰时，维生素A使肌内三酰甘油增加45%，而不影响整体脂肪。在三维培养体系中，RA通过激活VEGFA/VEGFR 2信号通路促进肌内SVF细胞毛细血管芽的发育，并促进随后的脂肪形成。在脂肪形成终末阶段，RA下调PPARγ、C/EBPα的表达，抑制脂质的积累。总之，维生素A/RA上调VEGFA并刺激肌内血管毛细血管发育，从而增加肌内脂肪祖细胞并有助于脂肪细胞形成。当在新生阶段给药时，维生素A促进牛肉大理石花纹的发育而不影响整体脂肪。
To explore the effects and underlying mechanism of vitamin A on beef marbling fat development, angus steers were injected vitamin A at birth and 1 month of age and in vitro experiments were performed to investigate the effects of retinoic acid (RA) on angiogenesis and adipogenesis of intramuscular stromal vascular (SVF) cells. Results showed that vitamin A administration increased the intramuscular PDGFRα+ adipose progenitors, improved adipogenic potential of intramuscular SVF cells and dramatically upregulated VEGFA. At slaughter, vitamin A increased intramuscular triacylglycerols by 45% without affecting overall fatness. In a 3D culture system, RA promoted capillary sprout development and promoted the subsequent adipogenesis of intramuscular SVF cells by activating VEGFA/VEGFR2 signaling. However, during terminal adipogenesis, RA downregulated PPARγ, C/EBPα and inhibited lipid accumulation. In conclusion, vitamin A/RA upregulate VEGFA and stimulate intramuscular vascular capillary development, which increases intramuscular adipose progenitors and contributes to adipocyte formation. When administrated at neonatal stage, vitamin A promotes beef marbling development without affecting overall fatness.