Directed proteomics identifies a plant-specific protein rapidly phosphorylated in response to bacterial and fungal elicitors.
Directed proteomics identifies a plant-specific protein rapidly phosphorylated in response to bacterial and fungal elicitors.
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DOI：
10.1105/tpc.13.6.1467
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发表时间：
2001-06
期刊：
影响因子：
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通讯作者：
S. Peck;T. Nühse;Daniel Hess;Alejandro Iglesias;Frederick Meins;Thomas Boller
中科院分区：
文献类型：
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作者：
S. Peck;T. Nühse;Daniel Hess;Alejandro Iglesias;Frederick Meins;Thomas Boller
对微生物信号分子的感知是植物进化出的策略的一部分，以抵御潜在病原体的攻击。为了更全面地了解这些反应中涉及的早期信号事件，我们使用放射性正磷酸盐脉冲标记拟南芥悬浮培养细胞，结合二维凝胶电泳和质谱法来鉴定快速磷酸化的蛋白质，以响应细菌和真菌的激发子。这些蛋白质之一，AtPhos43，以及番茄和水稻中的相关蛋白质，在用鞭毛蛋白或几丁质片段处理后几分钟内被磷酸化。通过测量从激发子处理的激素和防御反应突变体的提取物中免疫沉淀的AtPhos 43中的（32）P掺入，我们发现在鞭毛蛋白处理而不是几丁质处理后AtPhos 43的磷酸化依赖于FLS2，FLS2是一种参与鞭毛蛋白感知的受体样激酶。这两种诱导剂的诱导不依赖于水杨酸或EDS1，一种假定的脂肪酶参与防御信号。
The perception of microbial signal molecules is part of the strategy evolved by plants to survive attacks by potential pathogens. To gain a more complete understanding of the early signaling events involved in these responses, we used radioactive orthophosphate to pulse-label suspension-cultured cells of Arabidopsis in conjunction with two-dimensional gel electrophoresis and mass spectrometry to identify proteins that are phosphorylated rapidly in response to bacterial and fungal elicitors. One of these proteins, AtPhos43, and related proteins in tomato and rice, are phosphorylated within minutes after treatment with flagellin or chitin fragments. By measuring (32)P incorporation into AtPhos43 immunoprecipitated from extracts of elicitor-treated hormone and defense-response mutants, we found that phosphorylation of AtPhos43 after flagellin treatment but not chitin treatment is dependent on FLS2, a receptor-like kinase involved in flagellin perception. Induction by both elicitors is not dependent on salicylic acid or EDS1, a putative lipase involved in defense signaling.