Interleukin 2 and 15 activate Stat3α in human T lymphocytes
Interleukin 2 and 15 activate Stat3α in human T lymphocytes
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DOI：
10.1006/cyto.1998.0356
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发表时间：
1998-10-01
期刊：
影响因子：
3.8
通讯作者：
Odum, N
中科院分区：
文献类型：
--
作者：
Nielsen, M;Nordahl, M;Odum, N
信号转导器和转录激活剂 3 (Stat3) 最近被证明以两种选择性剪接异构体存在，一种是短形式 Stat3 beta，另一种是较长形式 Stat3 α，在转录活性方面表现出差异。目前尚不清楚哪种 Stat3 异构体会响应白细胞介素 (IL)-2 和 IL-15 而被激活。此处，使用细胞因子诱导的 Stat3 在先前激活的 CD4(+) 人 T 细胞中的激活进行了检查Stat3 抗体针对 Stat3 的不同区域。通过酪氨酸磷酸化、核易位和与 hSIE 寡核苷酸探针的结合确定，IL-2 和 IL-15 激活了缓慢迁移的异构体 Stat3 α。相反，观察到 Stat3 β 的激活很少或没有，这表明 IL-2 和 IL-15 主要激活人 CD4+ T 细胞中的 Stat3 α。通过这种方式，Stat3蛋白表达和激活的多样性可能提供调节细胞因子诱导的T细胞反应的额外手段。 (C) 1998 年学术出版社。
Signal transducer and activator of transcription 3 (Stat3) has recently been shown to exist in two alternatively spliced isoforms, a short form, Stat3 beta, and a longer form, Stat3 alpha, displaying differences in transcriptional activity, It is unknown which Stat3 isoform(s) is activated in response to interleukin (IL)-2 and IL-15, Here, cytokine-induced activation of Stat3 in previously activated CD4(+) human T cells was examined using Stat3 antibodies directed against different regions of Stat3. As determined by tyrosine phosphorylation, nuclear translocation and binding to an hSIE-oligonucleotide probe, IL-2 and IL-15 activated the slowly migrating isoform, Stat3 alpha. In contrast, minimal or no activation of Stat3 beta was observed, suggesting that IL-2 and IL-15 predominantly activate Stat3 alpha in human CD4(+) T cells. In this way, diversity in the expression and activation of Stat3 proteins may provide additional means of regulating cytokine-induced T cell responses. (C) 1998 Academic Press.