Orexins increase mRNA expressions of neurotrophin-3 in rat primary cortical neuron cultures
Orexins increase mRNA expressions of neurotrophin-3 in rat primary cortical neuron cultures
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DOI：
10.1016/j.neulet.2008.11.028
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发表时间：
2009-01-30
影响因子：
2.5
通讯作者：
Nakao, Kazuwa
中科院分区：
文献类型：
--
作者：
Yamada, Nobuko;Katsuura, Goro;Nakao, Kazuwa
食欲素和黑色素浓集激素（MCH）作为促食欲的神经肽存在于下丘脑外侧，其受体分布于大脑皮层和海马。在本研究中，食欲素-A，食欲素-B和MCH对神经营养素-3（NT-3）和脑源性神经营养因子（BDNF）的表达的调节作用，在原代皮层神经元培养使用定量实时PCR。Orexin-A和Orexin-B在0.01、0.1和1 μ M的浓度下暴露6天均显著增加NT-3 mRNA。Orexin-A和B在1 μ M时导致比对照增加两倍或更多。然而，没有这样的NT-s mRNA的增加发生与暴露于MCH在相同浓度的食欲素。BDNF的mRNA表达仅在1 μ M的食欲素-B作用下显著增加。这些结果表明，食欲素，但不是MCH，可能是一个诱导剂NT-3在大脑皮层。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Orexins and melanin-concentrating hormone (MCH) as orexigenic neuropeptides are present in the lateral hypothalamus, and their receptors are distributed in the cerebral cortex and hippocampus. In the present study, the regulatory effects of orexin-A, orexin-B and MCH on neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF) expressions were examined in primary cortical neuron cultures using quantitative real-time PCR. Both orexin-A and orexin-B on 6-day exposure significantly increased the NT-3 mRNA at concentrations of 0.01, 0.1 and 1 mu M. Orexin-A and B at 1 mu M led to an increase of twofold or more over the control. However, no such NT-s mRNA increase occurred with exposure to MCH at the same concentrations as orexins. The mRNA expression of BDNF was significantly increased only by orexin-B at 1 mu M. These findings suggest that orexins, but not MCH, may be an inducer of NT-3 in the cerebral cortex. (C) 2008 Elsevier Ireland Ltd. All rights reserved.