MALDI mass spectrometric imaging of lipids in rat brain injury models.
MALDI mass spectrometric imaging of lipids in rat brain injury models.
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DOI：
10.1007/s13361-011-0122-z
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发表时间：
2011-06
影响因子：
3.2
通讯作者：
Murphy RC
中科院分区：
文献类型：
--
作者：
Hankin JA;Farias SE;Barkley RM;Heidenreich K;Frey LC;Hamazaki K;Kim HY;Murphy RC
基质辅助激光解吸电离/成像质谱（MALDI IMS）与飞行时间分析仪被用来表征脂质分子种类在两种损伤模型大鼠脑中的分布。双侧颈动脉闭塞后大鼠脑的缺血/再灌注损伤改变了海马区，特别是CA 1区的磷脂的外观。这些脑区在m/z 548.5处的离子丰度也大幅增加，碰撞活化支持将该离子鉴定为由神经酰胺（d18：1/18：0）产生，神经酰胺是一种已知与细胞凋亡相关的脂质。通过MALDI IMS检查大鼠的创伤性脑损伤模型，并且损伤区域也显示神经酰胺（d18：1/18：0）的增加和最丰富的磷酸胆碱分子种类16：0/18：1（PC）的钾加合物的信号的显著损失，以及钠加合物离子的相应增加。PC碱性附着离子的这种变化被认为是可能通过损伤引起的Na/K-ATP酶损失引起的水肿和细胞外液内流的结果。这些研究揭示了MALDI IMS检查组织脂质生化变化的价值，并将提供最终了解组织损伤相关生化机制所需的数据。
Matrix-assisted laser desorption ionization/imaging mass spectrometry (MALDI IMS) with a time-of-flight analyzer was used to characterize the distribution of lipid molecular species in the brain of rats in two injury models. Ischemia/reperfusion injury of the rat brain after bilateral occlusion of the carotid artery altered appearance of the phospholipids present in the hippocampal region, specifically the CA1 region. These brain regions also had a large increase in the ion abundance at m/z 548.5 and collisional activation supported identification of this ion as arising from ceramide (d18:1/18:0), a lipid known to be associated with cellular apoptosis. Traumatic brain injury model in the rat was examined by MALDI IMS and the area of damage also showed an increase in ceramide (d18:1/18:0) and a remarkable loss of signal for the potassium adduct of the most abundant phosphocholine molecular species 16:0/18:1 (PC) with a corresponding increase in the sodium adduct ion. This change in PC alkali attachment ion was suggested to be a result of edema and influx of extracellular fluid likely through a loss of Na/K-ATPase caused by the injury. These studies reveal the value of MALDI IMS to examine tissues for changes in lipid biochemistry and will provide data needed to eventually understand the biochemical mechanisms relevant to tissue injury.