Experimental Phage Therapy for Burkholderia pseudomallei Infection.
Experimental Phage Therapy for Burkholderia pseudomallei Infection.
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DOI：
10.1371/journal.pone.0158213
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Vadivelu J
中科院分区：
文献类型：
--
作者：
Guang-Han O;Leang-Chung C;Vellasamy KM;Mariappan V;Li-Yen C;Vadivelu J
类鼻疽伯克霍尔德氏菌是一种对多种抗生素具有内在耐药性的革兰氏阴性菌。噬菌体已被开发用作替代治疗疗法，特别是用于对常规抗生素无反应的细菌感染。在这项研究中，我们研究了使用抗病毒药物治疗感染了B的细胞。假鼻疽从海水中分离的噬菌体C34进行纯化，并使用透射电子显微镜（TEM）的基础上，其主机范围和形态特征。噬菌体C34对B的裂解率为39.5%。类鼻疽临床菌株。由于存在收缩尾，噬菌体C34被分类为肌尾病毒科的成员，一种有尾双链DNA病毒。将2 × 105个A549细胞暴露于2 × 107 PFU的噬菌体C34中，24小时后再感染2 × 106 CFU的B。结果表明，细胞的存活率由未处理组的22.8 ± 6.0%提高到41.6 ± 6.8%。此外，噬菌体的应用成功地挽救了33.3%的感染B的小鼠。并显著降低了噬菌体处理的小鼠脾脏中的细菌负荷。这些发现表明噬菌体可能是B的潜在抗菌剂。类鼻疽感染。
Burkholderia pseudomallei is an intracellular Gram-negative bacterial pathogen intrinsically resistant to a variety of antibiotics. Phages have been developed for use as an alternative treatment therapy, particularly for bacterial infections that do not respond to conventional antibiotics. In this study, we investigated the use of phages to treat cells infected with B. pseudomallei. Phage C34 isolated from seawater was purified and characterised on the basis of its host range and morphology using transmission electron microscopy (TEM). Phage C34 was able to lyse 39.5% of B. pseudomallei clinical strains. Due to the presence of contractile tail, phage C34 is classified as a member of the family Myoviridae, a tailed double-stranded DNA virus. When 2 × 105 A549 cells were exposed to 2 × 107 PFU of phage C34, 24 hours prior to infection with 2 × 106 CFU of B. pseudomallei, it was found that the survivability of the cells increased to 41.6 ± 6.8% as compared to 22.8 ± 6.0% in untreated control. Additionally, application of phage successfully rescued 33.3% of mice infected with B. pseudomallei and significantly reduced the bacterial load in the spleen of the phage-treated mice. These findings indicate that phage can be a potential antimicrobial agent for B. pseudomallei infections.