A Novel Gene, fudoh, in the SCCmec Region Suppresses the Colony Spreading Ability and Virulence of Staphylococcus aureus
A Novel Gene, fudoh, in the SCCmec Region Suppresses the Colony Spreading Ability and Virulence of Staphylococcus aureus
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DOI：
10.1371/journal.pone.0003921
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发表时间：
2008-12-11
期刊：
影响因子：
3.7
通讯作者：
Sekimizu, Kazuhisa
中科院分区：
文献类型：
--
作者：
Kaito, Chikara;Omae, Yosuke;Sekimizu, Kazuhisa
金黄色葡萄球菌菌落可以在软琼脂板上扩散。我们比较了临床分离的甲氧西林敏感金黄色葡萄球菌（MSSA）和耐甲氧西林金黄色葡萄球菌（MRSA）的菌落扩散情况。所有MSSA菌株均表现出菌落扩散，但大多数携带SCCmec ii型的MRSA菌株（73%）几乎没有菌落扩散。从这些MRSA菌株中删除整个SCCmec ii型区域恢复了菌落传播。将一种由SCCmec ii型携带的新基因fudoh引入纽曼菌株抑制了菌落扩散。具有高传播能力的MRSA菌株（27%）没有fudoh或点突变的fudoh不抑制菌落传播。fudoh转化的Newman菌株减少了小鼠的外毒素产生并减弱了毒力。大多数社区获得性MRSA菌株携带SCCmec - iv型，不包括fudoh，具有较高的菌落传播能力。这些发现表明，SCCmec ii型区域的fudoh抑制菌落扩散和外毒素的产生，并参与金黄色葡萄球菌的发病机制。
Staphylococcus aureus colonies can spread on soft agar plates. We compared colony spreading of clinically isolated methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). All MSSA strains showed colony spreading, but most MRSA strains (73%) carrying SCCmec type-II showed little colony spreading. Deletion of the entire SCCmec type-II region from these MRSA strains restored colony spreading. Introduction of a novel gene, fudoh, carried by SCCmec type-II into Newman strain suppressed colony spreading. MRSA strains with high spreading ability (27%) had no fudoh or a point-mutated fudoh that did not suppress colony spreading. The fudoh-transformed Newman strain had decreased exotoxin production and attenuated virulence in mice. Most community-acquired MRSA strains carried SCCmec type-IV, which does not include fudoh, and showed high colony spreading ability. These findings suggest that fudoh in the SCCmec type-II region suppresses colony spreading and exotoxin production, and is involved in S. aureus pathogenesis.